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J.gamma1.WT
J.gamma1.WT
規格:
價格:
編號:B164868
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱
J.gamma1.WT
商品貨號
B164868
Organism
Homo sapiens, human
Cell Type
T lymphocyte
培養基
Product Format
frozen
Morphology
lymphoblast
Culture Properties
clusters in suspension
Biosafety Level
2  [Cells contain CMV and SV-40 viral DNA sequences]

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Disease
acute T cell leukemia
Gender
male
Applications
The J.gamma1.WT cell line was derived from the phospholipase C-gamma1 (PLC-gamma1) deficient J.gamma1 cell line (ATCC CRL-2678) by stable transfection with a wild-type expression vector, pcaPLC-gamma1.
The J.gamma1.WT derivative (ATCC CRL-2679) stably expresses PLC-gamma1at levels comparable to the Jurkat cell line.
Derivation
The J.gamma1.WT cell line was derived from the phospholipase C-gamma1 (PLC-gamma1) deficient J.gamma1 cell line (ATCC CRL-2678) by stable transfection with a wild-type expression vector, pcaPLC-gamma1.
The J.gamma1.WT derivative (ATCC CRL-2679) stably expresses PLC-gamma1at levels comparable to the Jurkat cell line.
Clinical Data
male
Comments
The J.gamma1.WT cell line was derived from the phospholipase C-gamma1 (PLC-gamma1) deficient J.gamma1 cell line (ATCC CRL-2678) by stable transfection with a wild-type expression vector, pcaPLC-gamma1.
The vector contains cytomegalovirus (CMV) and SV40 viral DNA sequences and the neomycin resistance gene.
The J.gamma1.WT derivative (ATCC CRL-2679) stably expresses PLC-gamma1at levels comparable to the Jurkat cell line.
Complete Growth Medium
RPMI 1640 medium with 2 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate, 4.5 g/L glucose, 10 mM HEPES and 1.0 mM sodium pyruvate and supplemented with 0.4 mg/ml G418, 90%; fetal bovine serum, 10%
Subculturing
Medium Renewal: Add fresh medium every 2 to 3 days (depending on cell density)
Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 3 X 10 exp5 viable cells/ml.
Maintain cell density between 3 X 10 exp5 and 2 X 10 exp6 viable cells/ml.
Cryopreservation
culture medium, 45%; fetal bovine serum, 45%; DMSO, 10%
Culture Conditions
Temperature: 37.0°C
Name of Depositor
RT Abraham
Deposited As
human
References

Irvin BJ, et al. Pleiotropic contributions of phospholipase C-gamma1 (PLC-gamma1) to T-cell antigen receptor-mediated signaling reconstitution studies of a PLC-gamma1-deficient Jurkat T-cell line. Mol. Cell. Biol. 20: 9149-9161, 2000. PubMed: 11094067

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