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JC
JC
規(guī)格:
價(jià)格:
編號(hào):B164888
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱
JC
商品貨號(hào)
B164888
Organism
Mus musculus, mouse
Tissue
mammary gland
培養(yǎng)基
Cell Type
Epithelial-like
Product Format
frozen
Morphology
epithelial
Culture Properties
adherent
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Disease
adenocarcinoma
Age
1.5 years
Gender
Female
Strain
BALB/cRos
Storage Conditions
liquid nitrogen vapor phase
Karyotype
modal number = 40; range = 26 to 100
Derivation
JC is an epithelial-like cell line established in 1983 from a spontaneous primary mammary adenocarcinoma along the milk line.
Clinical Data
female
The original tumor was passaged once in female BALB/c mice, and the resulting tumors were harvested and passaged in vitro for six months.
Tumorigenic
Yes
Effects
Yes, produces tumors with papillary adenocarcinoma morphology in BALB/c mice
Comments
Electron microscopic examination detected the presence of cellular tonofilaments and desmosomes.
No type B or C virus particles were detected, although intracisternal A particles were observed occasionally.
The original tumor was passaged once in female BALB/c mice, and the resulting tumors were harvested and passaged in vitro for six months.
Complete Growth Medium
The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing
Remove medium, rinse with 0.25% trypsin, 0.03% EDTA solution and remove trypsin. Add fresh trypsin solution and let the culture sit at room temperature (or at 37°C) until the cells detach. Add fresh medium, aspirate and dispense into new flasks.
Subcultivation Ratio: A subcultivation ratio of 1:4 to 1:6 is recommended
Medium Renewal: Twice per week
Cryopreservation
Culture medium, 95%; DMSO, 5%
Population Doubling Time
12 to 16 hrs
Name of Depositor
TM Chu
Deposited As
Mus musculus
Year of Origin
1983
References

Capone PM, et al. Immunotherapy in a spontaneously developed murine mammary carcinoma with syngeneic monoclonal antibody. Cancer Immunol. Immunother. 25: 93-99, 1987. PubMed: 3664534

Chao TY, Chu TM. Characterization of a new spontaneously developed murine mammary adenocarcinoma in syngeneic BALB/c hosts. In Vitro Cell. Dev. Biol. 25: 621-626, 1989. PubMed: 2666384

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