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JM1

規格:

價格:

編號:B164894

品牌:Mingzhoubio

標準菌株

定量菌液

DNA

RNA

規格:

凍干粉

斜面

甘油

平板

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產品名稱

JM1

商品貨號

B164894

Organism

Homo sapiens, human

Tissue

blood

培養基

Cell Type

pre-B lymphoblast

Product Format

frozen

Morphology

lymphoblast

Culture Properties

suspension

Biosafety Level

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Disease

B cell lymphoma

Storage Conditions

liquid nitrogen vapor phase

Disclosure

This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC.

Derivation

The JM1 cell line was established from cells taken from a patient with immunoblastic B cell lymphoma - leukemia.

Antigen Expression

HLA-DR; Homo sapiens, expressed

CD19; Homo sapiens, expressed

CD10; Homo sapiens, expressed

Genes Expressed

CD19; Homo sapiens, expressed ,CD10; Homo sapiens, expressed ,HLA-DR; Homo sapiens, expressed

Comments

The cells are negative for CD7, My 7, My 9 and surface IgM.

Complete Growth Medium

The base medium for this cell line is ATCC-formulated Iscove's Modified Dulbecco's Medium, Catalog No. 30-2005. To make the complete growth medium, add the following components to the base medium:

0.05 mM 2-mercaptoethanol
fetal bovine serum to a final concentration of 10%

Subculturing

Cultures can be maintained by addition or replacement of fresh medium. Alternatively, cultures may be established by centrifugation. Adherent cells can be dislodged by scraping. Initiate cultures at 2 to 5 x 10⁵ viable cells/mL. Subculture when cell density reaches 1 x 10⁶ cells/mL.

Medium Renewal: 2 to 3 times per week

Cryopreservation

Complete culture medium, 95%; DMSO 5%. Cell culture tested DMSO is available as ATCC^? Catalog No. 4-X.

Culture Conditions

Temperature: 37°C

Atmosphere: Air, 95%; Carbon dioxide (CO₂), 5%

STR Profile

D5S818: 12

D13S317: 11

D7S820: 8, 10

D16S539: 9

vWA: 17

THO1: 6

TPOX: 10, 11

CSF1PO: 12

Amelogenin: X Y

Name of Depositor

Immunex Corporation

Deposited As

Homo sapiens

U.S. Patent Number

5,976,860

References

Park LS, et al. Heterogeneity in human interleukin-3 receptors. A subclass that binds human granulocyte/macrophage colony stimulating factor. J. Biol. Chem. 264: 54205427, 1989. PubMed: 2647717

Hay, R. J., Caputo, J. L., and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC.

Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988.

Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC.

B240530：pJM128 plasmid in E. coli

B207167：CRI-JM14

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