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KB
KB
規(guī)格:
價格:
編號:B164903
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱
KB
商品貨號
B164903
Organism
Homo sapiens, human
Tissue
HeLa contaminant
培養(yǎng)基
Product Format
frozen
Morphology
epithelial
Culture Properties
adherent
Biosafety Level
2 [Cells contain human papilloma virus]

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Disease
Carcinoma, Papilloma
Applications
This cell line is a suitable transfection host.
Storage Conditions
liquid nitrogen vapor phase
Derivation
This line was originally thought to be derived from an epidermal carcinoma of the mouth, but was subsequently found, based on isoenzyme analysis, HeLa marker chromosomes, and DNA fingerprinting, to have been established via HeLa cell contamination.
HeLa Markers
Y
Genes Expressed
keratin
Comments
The cells are positive for keratin by immunoperoxidase staining.
KB cells have been reported to contain human papillomavirus 18 (HPV-18) sequences.
NOTE: Cells of this line contain HeLa marker chromosomes, and were derived via HeLa contamination
Complete Growth Medium
The base medium for this cell line is ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing
Remove medium, and rinse with 0.25% trypsin, 0.03% EDTA solution. Remove the solution and add an additional 1 to 2 mL of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37°C) until the cells detach. Add fresh culture medium, aspirate and dispense into new culture flasks.
Subcultivation Ratio: A subcultivation ratio of 1:4 to 1:10 is recommended
Medium Renewal: 2 to 3 times per week
Cryopreservation
Freeze medium: Complete growth medium, 95%; DMSO, 5%
Storage temperature: liquid nitrogen vapor temperature
Culture Conditions
Temperature: 37°C
STR Profile
Amelogenin: X
CSF1PO: 9,10
D13S317: 12,13.3
D16S539: 9,10
D5S818: 11,12
D7S820: 8,12
THO1: 7
TPOX: 8,12
vWA: 16,18
Isoenzymes
G6PD, A
Name of Depositor
H Eagle
Deposited As
Homo sapiens
References

Eagle H. Propagation in a fluid medium of a human epidermoid carcinoma, strain KB. Proc. Soc. Exp. Biol. Med. 89: 362-364, 1955. PubMed: 13254761

Boshart M, et al. A new type of papillomavirus DNA, its presence in genital cancer biopsies and in cell lines derived from cervical cancer. EMBO J. 3: 1151-1157, 1984. PubMed: 6329740

Salzman NP. Animal Cell Cultures. Science 133:1559-65. 1961 PubMed: 13745868

Eagle H, Foley GE. Cytotoxicity in human cell cultures as a primary screen for the detection of anti-tumor agents. Cancer Res. 18: 1017-1025, 1958. PubMed: 13596943

Foley GE, Handler AH. Differentiation of “Normal” and Neoplastic Cells Maintained in Tissue Culture by Implantation into Normal Hamsters. Proc Soc Exp Biol Med 94: 661-664 1957. PubMed: 13431913

Eagle H, et al. Viral susceptibility of a human carcinoma cell (strain KB). Proc. Soc. Exp. Biol. Med. 91: 361-364, 1956. PubMed: 13322936

St. Geme JW, et al. Characterization of the genetic locus encoding Haemophilus influenzae type b surface fibrils. J. Bacteriol. 178: 6281-6287, 1996. PubMed: 8892830

Chang K, Pastan I. Molecular cloning of mesothelin, a differentiation antigen present on mesothelium, mesotheliomas, and ovarian cancers. Proc. Natl. Acad. Sci. USA 93: 136-140, 1996. PubMed: 8552591

Lee RJ, Huang L. Folate-targeted, anionic liposome-entrapped polylysine-condensed DNA for tumor cell-specific gene transfer. J. Biol. Chem. 271: 8481-8487, 1996. PubMed: 8626549

Cross References

Nucleotide (GenBank) : AW062989 EST00001 PT-PCR products Homo sapiens cDNA clone K-5, mRNA sequence.

Nucleotide (GenBank) : AW062990 EST00002 PT-PCR products Homo sapiens cDNA clone K-13, mRNA sequence.

Nucleotide (GenBank) : AW062991 EST00003 PT-PCR products Homo sapiens cDNA clone K-16, mRNA sequence.

Nucleotide (GenBank) : AW062992 EST00004 PT-PCR products Homo sapiens cDNA clone K-32, mRNA sequence.

Nucleotide (GenBank) : AW062993 EST00005 PT-PCR products Homo sapiens cDNA clone K-33, mRNA sequence.

Nucleotide (GenBank) : AW062994 EST00006 PT-PCR products Homo sapiens cDNA clone K-34, mRNA sequence.

Nucleotide (GenBank) : AW062995 EST00007 PT-PCR products Homo sapiens cDNA clone K-45, mRNA sequence.

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