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KHOS/NP (R-970-5)
KHOS/NP (R-970-5)
規(guī)格:
價(jià)格:
編號(hào):B164911
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱
KHOS/NP (R-970-5)
商品貨號(hào)
B164911
Organism
Homo sapiens, human
Tissue
bone
培養(yǎng)基
Product Format
frozen
Culture Properties
adherent
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Disease
osteosarcoma
Age
13 years
Gender
female
Ethnicity
Caucasian
Applications
The cells are useful producing MSV pseudotypes with various ecotropic and xenotropic murine leukemia viruses.
Storage Conditions
liquid nitrogen vapor phase
Derivation
This cell line was derived from HOS (ATCC CRL-1543) by transformation using Kirsten murine sarcoma virus (Ki-MSV).
Clinical Data
female
Caucasian
13 years
Tumorigenic
Yes
Effects
Yes, Tumors developed within 21 days at 100% frequency (5/5) in nude mice inoculated subcutaneously with 10(7) cells.
Comments
The cells exhibit a high saturation density, a high plating efficiency in soft agar and produce tumors in nude mice.

Cells carry the Ki-MSV genome but do not produce infectious virus particles or viral antigens.

Complete Growth Medium
The base medium for this cell line is ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing
Remove medium, add fresh 0.25% trypsin solution for 2 to 3 minutes, remove trypsin and let the culture sit at room temperature until the cells detach. Add fresh medium, aspirate and dispense into new flasks.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:4 is recommended
Medium Renewal: 2 to 3 times per week
STR Profile
Amelogenin: X
CSF1PO: 12
D13S317: 12
D16S539: 10,13
D5S818: 13
D7S820: 11,12
THO1: 6
TPOX: 8,11
vWA: 18
Name of Depositor
JS Rhim
Deposited As
Homo sapiens
References

Rhim JS, et al. Non-producer human cells induced by murine sarcoma virus. Int. J. Cancer 15: 23-29, 1975. PubMed: 165148

Rhim JS, et al. Characterization of non-producer human cells induced by Kirsten sarcoma virus. Int. J. Cancer 16: 840-849, 1975. PubMed: 171229

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