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KL304
KL304
規格:
價格:
編號:B164916
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱
KL304
商品貨號
B164916
Organism
Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)
Cell Type
hybridoma: B lymphocyte
培養基
Product Format
frozen
Morphology
lymphoblast
Culture Properties
suspension
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Applications
Animals were immunized with synthetic peptide corresponding to residues 146 to 157 of the mouse As/beta protein (domain beta 1).
The antibody reacts with denatured beta chains of the a, f, k, j, s and bw haplotypes.
Storage Conditions
liquid nitrogen vapor phase
Derivation
Spleen cells were fused with Sp2/0-Ag14 myeloma cells.
Genes Expressed
immunoglobulin; monoclonal antibody; against the beta chain of mouse Class II antigens
Cellular Products
immunoglobulin; monoclonal antibody; against the beta chain of mouse Class II antigens
Comments
Animals were immunized with synthetic peptide corresponding to residues 146 to 157 of the mouse As/beta protein (domain beta 1).
Spleen cells were fused with Sp2/0-Ag14 myeloma cells.
The antibody reacts with denatured beta chains of the a, f, k, j, s and bw haplotypes.
It does not detect the intact H-2b Class II molecule, and does not detect the beta chain on the cell surface.
It does not react with molecules of the b, d, p or q haplotypes.
The reactive determinant most likely involves residues 153 through 155.
Complete Growth Medium
Dulbecco's modified Eagle's medium with 4 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate and 4.5 g/L glucose and supplemented with 1% nonessential amino acids, 10 mM HEPES, 0.01 mM 2-mercaptoethanol and 10% fetal bovine serum
Subculturing
Protocol: Cultures can be maintained by addition of fresh medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 1 X 10(5) viable cells/ml. Do not allow the cell concentration to exceed 1 X 10(6) cells/ml.
Subcultivation Ratio: Maintain cultures at a cell concentration between 1 X 10(5) and 1 X 10(6) cells/ml.
Medium Renewal: Every 2 to 3 days
Cryopreservation
Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO
Storage temperature: liquid nitrogen vapor phase
Culture Conditions
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37.0°C
Isotype
IgG2b
Name of Depositor
JA Frelinger
Deposited As
mouse (B cell); mouse (myeloma)
References

LaPan KE, et al. Production and characterization of two new mouse monoclonal antibodies reactive with denatured mouse class II beta chains. Hybridoma 11: 217-223, 1992. PubMed: 1376718

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