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KM703
KM703
規格:
價格:
編號:B164921
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱
KM703
商品貨號
B164921
Organism
Rattus norvegicus (B cell); Mus musculus (myeloma), rat (B cell); mouse (myeloma)
Cell Type
hybridoma: B lymphocyte
培養基
Product Format
frozen
Morphology
lymphoblast
Culture Properties
suspension
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Applications
Animals were immunized with the BMS2 bone marrow derived stromal cell line.
KM703 recognizes an epitope unrelated to those detected by KM81, KM114 and KM201 (see ATCC TIB-241, ATCC TIB-242 and ATCC TIB-240).
Derivation
Animals were immunized with the BMS2 bone marrow derived stromal cell line.
Spleen cells were fused with Sp2/0 myeloma cells.
Genes Expressed
immunoglobulin; monoclonal antibody; against the functional domain of mouse Pgp-1 (CD44)
Cellular Products
immunoglobulin; monoclonal antibody; against the functional domain of mouse Pgp-1 (CD44)
Tumorigenic
Yes
Effects
Yes, form ascites in pristane primed CB.17.SCID mice
Comments
Animals were immunized with the BMS2 bone marrow derived stromal cell line.
Spleen cells were fused with Sp2/0 myeloma cells.
The antibody weakly blocks adhesion of a B cell hybridoma (BM-2) to BMS2 cells or to hyaluronate coated dishes.
The antibody prevents growth of lymphoid and myeloid cells in long term bone marrow cultures.
KM703 recognizes an epitope unrelated to those detected by KM81, KM114 and KM201 (see ATCC TIB-241, ATCC TIB-242 and ATCC TIB-240).
Complete Growth Medium
RPMI 1640 medium with 0.05 mM 2-mercaptoethanol, 85%; fetal bovine serum, 15%
Subculturing
Medium Renewal: Every 2 to 4 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 1 x 10 exp5 cells/ml and maintain between 1 x 10 exp5 and 1 x 10 exp6 cells/ml.
Isotype
IgG2a
Name of Depositor
PW Kincade
Deposited As
rat (B cell); mouse (myeloma)
References

Miyake K, et al. Monoclonal antibodies to Pgp-1/CD44 block lympho-hemopoiesis in long-term bone marrow cultures. J. Exp. Med. 171: 477-488, 1990. PubMed: 2406365

Miyake K, et al. Hyaluronate can function as a cell adhesion molecule and CD44 participates in hyaluronate recognition. J. Exp. Med. 172: 69-75, 1990. PubMed: 2193100

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