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當(dāng)前位置: 首頁 > ATCC代理 > KMC8.8 B164924
KMC8.8
KMC8.8
規(guī)格:
價格:
編號:B164924
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱
KMC8.8
商品貨號
B164924
Organism
Rattus norvegicus (B cell); Mus musculus (myeloma), rat (B cell); mouse (myeloma)
Cell Type
hybridoma: B lymphocyte
培養(yǎng)基
Product Format
frozen
Morphology
lymphoblast
Culture Properties
suspension
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Applications
The antibody reacts with mouse CD9 (expressed by bone marrow stromal cells, megakaryocytes, platelets, myeloid cells and subpopulations of mature lymphocytes.
Derivation
Spleen cells were fused with Sp2/0-Ag14 myeloma cells.
Genes Expressed
immunoglobulin; monoclonal antibody; against mouse CD9
Cellular Products
immunoglobulin; monoclonal antibody; against mouse CD9
Tumorigenic
Yes
Effects
Yes, produces only modest amounts of ascites in SCID mice because it usually fatal within two weeks of inoculation
Comments
Animals were immunized with BMS2 murine bone marrow stromal cells.
Spleen cells were fused with Sp2/0-Ag14 myeloma cells.
The antibody reacts with mouse CD9 (expressed by bone marrow stromal cells, megakaryocytes, platelets, myeloid cells and subpopulations of mature lymphocytes.
Complete Growth Medium
The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, ATCC 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum (ATCC 30-2020) to a final concentration of 10%.
Subculturing
Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
Cryopreservation
Culture medium, 95%; DMSO, 5%
Isotype
IgG2a
Name of Depositor
PW Kincade
Deposited As
rat (B cell); mouse (myeloma)
References

Oritani K, et al. Antibody ligation of CD9 modifies production of myeloid cells in long-term cultures. Blood 87: 2252-2261, 1996. PubMed: 8630385

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