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KU812

規格:

價格:

編號:B164927

品牌:Mingzhoubio

標準菌株

定量菌液

DNA

RNA

規格:

凍干粉

斜面

甘油

平板

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產品名稱

KU812

商品貨號

B164927

Organism

Homo sapiens, human

Tissue

peripheral blood

培養基

Cell Type

basophil

Product Format

frozen

Morphology

myeloblast

Culture Properties

suspension

Biosafety Level

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Disease

chronic myelogenous leukemia (CML)

Age

38 years adult

Gender

male

Ethnicity

Japanese

Storage Conditions

liquid nitrogen vapor phase

Karyotype

58, XY, t(9;22)(q34;q11), i(17q), +1, +4, +6, +6, +8, +der(9)t(9;22), + der(9)t(9;22), +der(11)q(11), +14, +15, +19, +21, +21, -9 Ref

Kishi K. A new leukemia cell line with Philadelphia chromosome characterized as basophil precursors. Leuk. Res. 9: 381-390, 1985. PubMed: 3858609

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Derivation

The KU812 cell line was established from the peripheral blood of a patient in blast crisis of chronic myelogenous leukemia.

Clinical Data

male

38 years adult

Japanese

Antigen Expression

CD3; Homo sapiens

alanyl (membrane) aminopeptidase (ANPEP, CD13); Homo sapiens

Comments

The cells contain at least one Ph1 (Philadelphia) chromosome. The cell line has some characteristics of basophilic leukocytes (Fc receptors, basophilic granules, histamine production), and are negative for lymphoid markers.

The cell line has some characteristics of basophilic leukocytes (Fc receptors, basophilic granules, histamine production), and are negative for lymphoid markers.

Complete Growth Medium

The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, ATCC 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum (ATCC 30-2020) to a final concentration of 10%.

Subculturing

Cultures can be maintained by addition of fresh medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 3 X 10⁵ viable cells/mL. Do not allow the cell concentration to exceed 3 x 10⁶ cells/mL.

Medium Renewal: every 2 to 3 days

Cryopreservation

Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO

Storage temperature: liquid nitrogen vapor phase

Culture Conditions

Atmosphere: air, 95%; carbon dioxide (CO₂), 5%

Temperature: 37°C

Population Doubling Time

20 to 30 hours

Name of Depositor

K Kishi

Year of Origin

August 1, 1981

References

Kishi K. A new leukemia cell line with Philadelphia chromosome characterized as basophil precursors. Leuk. Res. 9: 381-390, 1985. PubMed: 3858609

Okano H, et al. Hemoglobin synthesis of both adult and fetal types in a human CML cell line. J. Biochem. 104: 162-164, 1988. PubMed: 2460439

Okamura S, et al. Expression of cytokine genes in hematological malignancies. Nippon Ketsueki Gakkai Zasshi 52: 1423-1432, 1989. PubMed: 2698048

The KU812 cell line was established from the peripheral blood of a patient in blast crisis of chronic myelogenous leukemia. The cells contain at least one Ph1 (Philadelphia) chromosome. The cell line has some characteristics of basophilic leukocytes (Fc receptors, basophilic granules, histamine production), and are negative for lymphoid markers.

Blom T, et al. Phenotypic characterization of KU812, a cell line identified as an immature human basophilic leukocyte. Eur. J. Immunol. 22: 2025-2032, 1992. PubMed: 1639103

Kishi K. A new leukemia cell line with Philadelphia chromosome characterized as basophil precursors. Leuk. Res. 9: 381-390, 1985. PubMed: 3858609

B164929：KU812F [KU-812-F]

B164928：KU812E

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