Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Strain
Strain: ? (B cell); BALB/c (myeloma)
Applications
The antibody reacts with the M-23 antigen system.
Disclosure
This material is cited in a US or other Patent and may not be used to
infringe the claims. Depending on the wishes of the Depositor, ATCC may be
required to inform the Patent Depositor of the party to which the material was
furnished. This material may not have been produced or characterized by ATCC.
Genes Expressed
immunoglobulin; monoclonal antibody; against a human melanoma cell line (M-23 antigen system)
Cellular Products
immunoglobulin; monoclonal antibody; against a human melanoma cell line (M-23 antigen system)
Comments
Animals were immunized with the SK-MEL-33 human melanoma cell line.
The antibody precipitates two glycoproteins (90000 and 150000 daltons)from extracts of SK-MEL-19 cells.
It reacted with all melanoma cell lines tested (n = 20), fetal and adult cultured melanocytes and various normal and neoplastic tissues.
The antibody reacts with the M-23 antigen system.
Complete Growth Medium
RPMI 1640 medium with 2 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate, 4.5 g/L glucose, 10 mM HEPES, 1.0 mM sodium pyruvate supplemented with 0.1 mM nonessential amino acids, 90%; fetal bovine serum, 10%
Subculturing
Medium Renewal: Add fresh medium every 2 to 3 days (depending on cell density)
Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 1 to 2 X 10 exp5 viable cells/ml.
Maintain cell density between 1 X 10 exp5 and 1 X 10 exp6 viable cells/ml.
