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當(dāng)前位置: 首頁(yè) > ATCC代理 > L368 B164944
L368
L368
規(guī)格:
價(jià)格:
編號(hào):B164944
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱
L368
商品貨號(hào)
B164944
Organism
Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)
Cell Type
hybridoma: B lymphocyte
培養(yǎng)基
Product Format
frozen
Morphology
lymphoblast
Culture Properties
suspension
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Applications
Tested and found negative for ectromelia virus (mousepox).
Derivation
Spleen cells were fused with NS-1 myeloma cells.
Genes Expressed
immunoglobulin; monoclonal antibody; against human beta-2-microglobulin (beta 2 microglobulin)
Cellular Products
immunoglobulin; monoclonal antibody; against human beta-2-microglobulin (beta 2 microglobulin)
Comments
Animals were immunized with the RPMI 8866 human B cell line.
Spleen cells were fused with NS-1 myeloma cells.
The antibody does not cross-react with mouse, rat, cat or monkey beta-2-microglobulin.
The antibody is not cytotoxic.
Tested and found negative for ectromelia virus (mousepox).
Complete Growth Medium
Dulbecco's modified Eagle's medium with 4.5 g/L glucose, 0.1 mM hypoxanthine and 0.016 mM thymidine, 90%; fetal bovine serum, 10%
Subculturing
Medium Renewal: Every 3 to 4 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
Isotype
IgG1; kappa light chain
Name of Depositor
LA Lampson
Deposited As
mouse (B cell); mouse (myeloma)
References

Lampson LA, et al. Striking paucity of HLA-A, B, C and beta2-microglobulin on human neuroblastoma cell lines. J. Immunol. 130: 2471-2478, 1983. PubMed: 6187860

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