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L5.1
L5.1
規格:
價格:
編號:B164945
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱
L5.1
商品貨號
B164945
Organism
Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)
Cell Type
hybridoma: B lymphocyte
培養基
Product Format
frozen
Morphology
lymphoblast
Culture Properties
suspension
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Disease
leukemia
Strain
Strain: BALB/c (B cell); BALB/c (myeloma)
Applications
The antibody reacts strongly with all morphologically recognizable precursors of the human erythroid lineage.
Tested and found negative for ectromelia virus (mousepox).
Derivation
Spleen cells were fused with P3X63Ag8.653 myeloma cells.
Genes Expressed
immunoglobulin; monoclonal antibody; against human transferrin receptor
Cellular Products
immunoglobulin; monoclonal antibody; against human transferrin receptor
Comments
Animals were immunized with the HL-60 promyelocytic leukemia cell line.
Spleen cells were fused with P3X63Ag8.653 myeloma cells.
The antibody precipitates an 87000 dalton glycoprotein present on the surface of K562 leukemia cells.
The antibody reacts strongly with all morphologically recognizable precursors of the human erythroid lineage.
Tested and found negative for ectromelia virus (mousepox).
Complete Growth Medium
The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 20%.
Subculturing
Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 1 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
Cryopreservation
culture medium 95%; DMSO, 5%
Culture Conditions
Temperature: 37.0°C
Isotype
IgG2a
Name of Depositor
G Rovera
Deposited As
mouse (B cell); mouse (myeloma)
References

Lebman D, et al. A monoclonal antibody that detects expression of transferrin receptor in human erythroid precursor cells. Blood 59: 671-678, 1982. PubMed: 7059675

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