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L5178-R (LY-R)
L5178-R (LY-R)
規(guī)格:
價(jià)格:
編號:B164946
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱
L5178-R (LY-R)
商品貨號
B164946
Organism
Mus musculus, mouse
Tissue
thymus
培養(yǎng)基
Cell Type
chemically induced
Product Format
frozen
Morphology
lymphoblast
Culture Properties
suspension
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Disease
lymphoma
Strain
DBA/2
Applications
The cells are resistant to X-rays, but sensitive to ultraviolet radiation, heat and some potential anti neoplastic drugs (e.
The X-ray sensitive L5178-S line (ATCC CRL-1723) was derived from this line.
Tested and found negative for ectromelia virus.
Derivation
This cell line was derived from the L5178 thymic lymphoma induced by methylcholanthrene in a DBA/2 mouse.
The X-ray sensitive L5178-S line (ATCC CRL-1723) was derived from this line.
Tumorigenic
Yes
Effects
Yes, in DBA/2 mice
Comments
The cells are resistant to X-rays, but sensitive to ultraviolet radiation, heat and some potential anti neoplastic drugs (e.g., cis-PAD).
After 6 months to 3 years in culture, the line converts to an X-ray sensitive state.
Tested and found negative for ectromelia virus.
Complete Growth Medium
Fischer's medium with 0.1 g/L sodium pyruvate and 1.125 g/L sodium bicarbonate, 90%; horse serum, 10%
Subculturing
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 4 x 10 4 cells/mL and maintain between 3 x 10 4 and 7 x 10 4 cells/mL.
Medium Renewal: Twice per week
Cryopreservation
culture medium, 90%; DMSO, 10%
Name of Depositor
JZ Beer
Deposited As
Mus musculus
References

Beer JZ, et al. X-ray and UV mutagenesis in two L5178Y cell strains differing in tumorigenicity, radiosensitivity, and DNA repair. Br. J. Cancer Suppl. 6: 107-111, 1984. PubMed: 6582899

Beer JZ, et al. Loss of tumorigenicity with simultaneous changes in radiosensitivity and photosensitivity during in vitro growth of L5178Y murine lymphoma cells. Cancer Res. 43: 4736-4742, 1983. PubMed: 6883332

Beer JZ, et al. Effects of low dose rate (0.003-0.025 Gy/h) chronic X-irradiation on radioresistant and radiosensitive L5178Y mouse lymphoma cells. Int. J. Radiat. Biol. 48: 609-619, 1985. PubMed: 3876312

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