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LB 27.4 (LB 15.13)
LB 27.4 (LB 15.13)
規格:
價格:
編號:B164958
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱
LB 27.4 (LB 15.13)
商品貨號
B164958
Organism
Mus musculus (B cell); Mus musculus (lymphoma), mouse (B cell); mouse (lymphoma)
Cell Type
hybridoma: B lymphocyte
培養基
Product Format
frozen
Morphology
lymphoblast
Culture Properties
suspension
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Disease
Lymphoma
Applications
The line was produced by fusing A20.2J lymphoma cells (I-Ad, I-Ed) with T cell depleted spleen cells from C57BL/10 mice (I-Ab, I-Eb).
Tested and found negative for ectromelia virus (mousepox).
Derivation
The line was produced by fusing A20.2J lymphoma cells (I-Ad, I-Ed) with T cell depleted spleen cells from C57BL/10 mice (I-Ab, I-Eb).
Comments
The LB 27.4 bears surface I-Ad,b/d,b and I-Ed,d/b molecules and can present antigen to appropriate I region restricted T cell hybridomas.
The line was produced by fusing A20.2J lymphoma cells (I-Ad, I-Ed) with T cell depleted spleen cells from C57BL/10 mice (I-Ab, I-Eb).
Tested and found negative for ectromelia virus (mousepox).
Complete Growth Medium
The base medium for this cell line is ATCC Hybri-Care Medium, Catalog No. 46-X. Hybri-Care Medium is supplied as a powder and should be reconstituted in 1 L cell-culture-grade water. To make the complete growth medium, add the following components to the base medium:
  • fetal bovine serum to a final concentration of 10%
  • 1.5 g/L sodium bicarbonate for use with 5% CO2 in air atmosphere
  • 0.05 mM 2-Mercaptoethanol

Subculturing
Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 1 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
Cryopreservation
Freeze medium: complete growth medium, 95%; DMSO, 5%
Culture Conditions
Temperature: 37.0°C
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Name of Depositor
P Marrack, J Kappler
Deposited As
mouse (B cell); mouse (lymphoma)
References

Kappler J, et al. Antigen presentation by Ia+ B cell hybridomas to H-2 restricted T cell hybridomas. Proc. Natl. Acad. Sci. USA 79: 3604-3607, 1982. PubMed: 6980413

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