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LCL 8664
LCL 8664
規(guī)格:
價格:
編號:B164966
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱
LCL 8664
商品貨號
B164966
Organism
Macaca mulatta, monkey, rhesus
Cell Type
B lymphocyte
培養(yǎng)基
Product Format
frozen
Morphology
lymphoblast
Culture Properties
suspension
Biosafety Level
2 Cells Contain HERPESVIRUS

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Disease
lymphoma
Age
5 years
Gender
male
Storage Conditions
liquid nitrogen vapor phase
Clinical Data
male
Genes Expressed
B lymphotropic herpesvirus
Cellular Products
B lymphotropic herpesvirus
Comments

CAUTION: the cells are infected with and produce a B lymphotropic herpesvirus (RhEBV) and may represent a potential biohazard.

Free RhEBV is present in the supernatant, and rhesus B lymphocyte transforming activity is present in the medium.

About 1% of the cells express virus capsid antigen, and all express the virus related nuclear antigen.

Complete Growth Medium
The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 4 x 105 cells/mL and maintain between 4 x 105 and 2 x 106 cells/mL.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:3 is recommended
Medium Renewal: 2 to 3 times per week
Cryopreservation
Culture medium, 95%; DMSO, 5%
Name of Depositor
SR Rangan
Deposited As
Macaca mulatta
References

Rangan SR, et al. Epstein-Barr virus-related herpesvirus from a rhesus monkey (Macaca mulatta) with malignant lymphoma. Int. J. Cancer 38: 425-432, 1986. PubMed: 3017870

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