欧美色淫,久久久三级,天天插天天干

Lec1 [originally named Pro-5WgaRI3C]

規格:

價格:

編號:B164968

品牌:Mingzhoubio

標準菌株

定量菌液

DNA

RNA

規格:

凍干粉

斜面

甘油

平板

加入購物車

在線咨詢收藏

產品名稱

Lec1 [originally named Pro-5WgaRI3C]

商品貨號

B164968

Organism

Cricetulus griseus, hamster, Chinese

Tissue

ovary

培養基

Product Format

frozen

Morphology

epithelial

Culture Properties

loosely adherent

Biosafety Level

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Gender

female

Applications

This cell line is a suitable transfection host.

Storage Conditions

liquid nitrogen vapor phase

Derivation

This line is a mutant clone derived from the parental CHO clone Pro-5 (a proline auxotroph, see ATCC CRL-1781) by selection for resistance to wheat germ agglutinin.

Clinical Data

female

Comments

The cells lack GlcNAc glycosyl transferase so that N-linked carbohydrates are blocked at the Man5-GlcNAC2-Asn intermediate. The line may be useful for studying the effects of altered N-linked glycosylation on the function and compartmentalization of endogenous glycoproteins or glycoproteins introduced by viral infection or transfection of foreign DNA.
The population contains Pro+ revertants at low frequency (approximately 1 in 100,000 cells), and should be suitable for complementation studies which require the Pro- marker.

The cells are proline auxotrophs and must be grown in a medium that contains proline (40 mg/L).

The population contains Pro+ revertants at low frequency (approximately 1 in 100000 cells), and should be suitable for complementation studies which require the Pro- marker.

Complete Growth Medium

Alpha minimum essential medium with ribonucleosides and deoxyribonucleosides, 90%; fetal bovine serum, 10%

Subculturing

Remove medium, and rinse with 0.25% trypsin, 0.03% EDTA solution. Remove the solution and add an additional 1 to 2 mL of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37°C) until the cells detach. Add fresh culture medium, aspirate and dispense into new culture flasks.

Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:4 is recommended

Medium Renewal: 2 to 3 times per week

Cryopreservation

Freeze medium: Complete growth medium 95%; DMSO, 5%

Storage temperature: liquid nitrogen vapor phase

Culture Conditions

Temperature: 37°C

Name of Depositor

P Stanley

Deposited As

Cricetulus griseus

References

Stanley P, et al. Selection and characterization of eight phenotypically distinct lines of lectin-resistant Chinese hamster ovary cells. Cell 6: 121-128, 1975. PubMed: 1182798

Stanley P, et al. Chinese hamster ovary cells selected for resistance to the cytotoxicity of phytohemagglutinin are deficient in a UDP-N-acetylglucosamine-- glycoprotein N-acetylglucosaminyltransferase activity. Proc. Natl. Acad. Sci. USA 72: 3323-3327, 1975. PubMed: 1059116

Cross References

Nucleotide (GenBank) : U65792 Cricetulus griseus mutant N-acetylglucosaminyltransferase I mRNA, complete cds.

王經理

手機號：17280875617

QQ：1438578920
胡經理

手機號：13345964880

QQ：2438244627
周經理

手機號：17757487661

QQ：1296385441
于經理

手機號：18067160830

QQ：2088210172
沈經理

手機號：19548299266

QQ：2662369050
李經理

手機號：13626845108

QQ：972239479