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LM2/1.6.11

規格:

價格:

編號:B164991

品牌:Mingzhoubio

標準菌株

定量菌液

DNA

RNA

規格:

凍干粉

斜面

甘油

平板

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產品名稱

LM2/1.6.11

商品貨號

B164991

Organism

Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)

Tissue

spleen

培養基

Cell Type

hybridoma: B lymphocyte

Product Format

frozen

Morphology

lymphoblast

Culture Properties

suspension

Biosafety Level

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Applications

The antibody reacts with the human Mac-1 antigen and is specific for the alpha chain.

The depositor states that the cell line should not be maintained in culture continuously for greater than four months or antibody production may be lost.

Cloning and freezing of the cells after minimal passage (with selection of positive clones) will prevent loss of antibody producing cells.

Tested and found negative for ectromelia virus (mousepox).

Storage Conditions

liquid nitrogen vapor phase

Derivation

Spleen cells were fused with P3X63Ag8.653 myeloma cells.

Genes Expressed

immunoglobulin; monoclonal antibody; against human Mac-1 antigen; against CD11b

Cellular Products

immunoglobulin; monoclonal antibody; against human Mac-1 antigen; against CD11b

Comments

Animals were immunized with immunoprecipitates of human granulocyte lysates using TS1/18 (see ATCC HB-203) and boosted with live human granulocytes.

Spleen cells were fused with P3X63Ag8.653 myeloma cells.

The antibody reacts with the human Mac-1 antigen and is specific for the alpha chain.

The depositor states that the cell line should not be maintained in culture continuously for greater than four months or antibody production may be lost.

Cloning and freezing of the cells after minimal passage (with selection of positive clones) will prevent loss of antibody producing cells.

Tested and found negative for ectromelia virus (mousepox).

Complete Growth Medium

The base medium for this cell line is ATCC Hybri-Care Medium, Catalog No. 46-X. Hybri-Care Medium is supplied as a powder and should be reconstituted in 1 L cell-culture-grade water and supplemented with 1.5 g/L sodium bicarbonate. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.

Subculturing

Protocol: Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 1 X 10(5) viable cells/ml.

Subcultivation Ratio: Maintain cell density between 1 X 10(5) and 1 X 10(6) viable cells/ml.

Medium Renewal: Every 2 to 3 days

Cryopreservation

Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO

Storage temperature: liquid nitrogen vapor phase

Culture Conditions

Temperature: 37.0°C

Isotype

mouse IgG1

Name of Depositor

LJ Miller, TA Springer

Deposited As

mouse (B cell); mouse (myeloma)

Passage History

Cloning and freezing of the cells after minimal passage (with selection of positive clones) will prevent loss of antibody producing cells.

References

Miller LJ, et al. Regulated expression of the Mac-1, LFA-1, p150,95 glycoprotein family during leukocyte differentiation. J. Immunol. 137: 2891-2900, 1986. PubMed: 2428876

Zhang L, Plow EF. Overlapping, but not identical, sites are involved in the recognition of C3bi, neutrophil inhibitory factor, and adhesvie ligands by the alpha M beta 2 integrin. J. Biol. Chem. 271: 18211-18216, 1996. PubMed: 8663418

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