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Lp1 MAB 3
Lp1 MAB 3
規格:
價格:
編號:B165003
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱
Lp1 MAB 3
商品貨號
B165003
Organism
Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)
Cell Type
hybridoma: B lymphocyte
培養基
Product Format
frozen
Morphology
lymphoblast
Culture Properties
suspension
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Applications
The antibody reacts with a surface antigen on 2 of the 10 antigenic subgroups of L. pneumophila.
and it is useful in clinical diagnostic tests for subtyping L. pneumophila serogroup 1 isolates for epidemiologic studies using indirect immunofluorescence assays.
Tested and found negative for ectromelia virus (mousepox).
Derivation
Spleen cells were fused with Sp2/0-Ag14 myeloma cells.
Genes Expressed
immunoglobulin; monoclonal antibody; against Legionella pneumophila serogroup 1
Cellular Products
immunoglobulin; monoclonal antibody; against Legionella pneumophila serogroup 1
Comments
Animals were immunized with the bacterial antigenic subgroup Knoxville 1 (ATCC 33153).
Spleen cells were fused with Sp2/0-Ag14 myeloma cells.
The antibody reacts with a surface antigen on 2 of the 10 antigenic subgroups of L. pneumophila.
and it is useful in clinical diagnostic tests for subtyping L. pneumophila serogroup 1 isolates for epidemiologic studies using indirect immunofluorescence assays.
Tested and found negative for ectromelia virus (mousepox).
Complete Growth Medium
The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 20%.
Subculturing
Medium Renewal: Every 2 to 4 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
Isotype
IgG2b
Name of Depositor
RM McKinney
Deposited As
mouse (B cell); mouse (myeloma)
References

Joly JR, et al. Development of a standard subgrouping scheme for Legionella pneumophila serogroup 1 using monoclonal antibodies. J. Clin. Microbiol. 23: 768-771, 1986. PubMed: 3517064

McKinney RM, et al. Monoclonal antibodies to Legionella pneumophila serogroup 1: possible applications in diagnostic tests and epidemiologic studies. Zentralbl. Bakteriol. Mikrobiol. Hyg. I Abt. Orig. A255: 91-95, 1983.

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