Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
In 1950, a primary colon tumor was resected from the patient (41 years old at that time). 24 years later a second primary tumor classified as a Dukes' type B mucin producing colon adenocarcinoma was excised and the cells were cultured.
A third primary tumor was discovered in 1980 in an area just proximal to the colostomy and was resected.
The established cell line is aneuploid, releases small amounts of carcinoembryonic antigen, fails to grow progressively in nude mice, and forms colonies only in enriched semisolid medium developed for tumor stem cells.
In the chick embryonic skin assay, the cells grow as a well differentiated abnormal colonic epithelium with little mitotic activity and low invasiveness.
The plating efficiency is 12% in the medium recommended below.
Rutzky LP, et al. Characterization of a human colonic adenocarcinoma cell line, LS 123. In Vitro 19: 99-107, 1983. PubMed: 6826200
