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LS-136
LS-136
規(guī)格:
價格:
編號:B165011
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱
LS-136
商品貨號
B165011
Organism
Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)
Cell Type
hybridoma: B lymphocyte
培養(yǎng)基
Product Format
frozen
Morphology
lymphoblast
Culture Properties
suspension
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Applications
The antibody binds to the MOPC 104E, J 558 and HOPC 1 myeloma proteins but not to the MOPC 315 myeloma protein.
The antibody is specific for the C1 domain.
Tested and found negative for ectromelia virus (mousepox).
Derivation
Animals were immunized with purified B1-8 monoclonal antibody.
Spleen cells were fused with P3X63Ag8 myeloma cells.
Genes Expressed
immunoglobulin; monoclonal antibody; against mouse lambda 1 light chain
Cellular Products
immunoglobulin; monoclonal antibody; against mouse lambda 1 light chain
Comments
Animals were immunized with purified B1-8 monoclonal antibody.
Spleen cells were fused with P3X63Ag8 myeloma cells.
The antibody binds to the MOPC 104E, J 558 and HOPC 1 myeloma proteins but not to the MOPC 315 myeloma protein.
The antibody is specific for the C1 domain.
Tested and found negative for ectromelia virus (mousepox).
Complete Growth Medium
The base medium for this cell line is ATCC Hybri-Care Medium, Catalog No. 46-X. Hybri-Care Medium is supplied as a powder and should be reconstituted in 1 L cell-culture-grade water and supplemented with 1.5 g/L sodium bicarbonate. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing
Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 1 x 10 exp5 cells/ml ay maintain between 1 x 10 exp5 and 1 x 10 exp6 cells/ml.
Isotype
IgG1; kappa light chain
Name of Depositor
K Rajewsky
Deposited As
mouse (B cell); mouse (myeloma)
References

Reth M, et al. Analysis of the repertoire of anti-(4-hydroxy-3-nitro-phenyl) acetyl (NP) antibodies in C57 BL/6 mice by cell fusion. II. Characterization of idiotopes by monoclonal anti-idiotope antibodies. Eur. J. Immunol. 9: 1004-1013, 1979. PubMed: 398773

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