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當(dāng)前位置: 首頁 > ATCC代理 > M17/4.4.11.9 (new clone of M17/4.2) B165042
M17/4.4.11.9 (new clone of M17/4.2)
M17/4.4.11.9 (new clone of M17/4.2)
規(guī)格:
價(jià)格:
編號(hào):B165042
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱
M17/4.4.11.9 (new clone of M17/4.2)
商品貨號(hào)
B165042
Organism
Rattus norvegicus (B cell); Mus musculus (myeloma), rat (B cell); mouse (myeloma)
Tissue
spleen
培養(yǎng)基
Cell Type
hybridoma: B lymphocyte
Product Format
frozen
Morphology
lymphoblast
Culture Properties
suspension
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Applications
Monoclonal antibodies against LFA-1 can block killing by inhibiting the adhesion between cytolytic T cells and target cells.
The LFA-1 and Mac-1 antigens are composed of two chains or subunits, alpha and beta.
Storage Conditions
liquid nitrogen vapor phase
Derivation
Spleen cells were fused with NS-1 myeloma cells.
The line was recloned by TA Springer in 1988, and a new seed stock was created at the ATCC.
Genes Expressed
immunoglobulin; monoclonal antibody; against the alpha subunit of LFA-1 (mouse cytotoxic T cell antigen, CD11a)
Cellular Products
immunoglobulin; monoclonal antibody; against the alpha subunit of LFA-1 (mouse cytotoxic T cell antigen, CD11a)
Comments
Animals were immunized with cytotoxic T cells from C57BL/6 mice immunized with P815 cells.
Spleen cells were fused with NS-1 myeloma cells.
LFA-1 is a cell surface antigen found on cytolytic T lymphocytes.
Monoclonal antibodies against LFA-1 can block killing by inhibiting the adhesion between cytolytic T cells and target cells.
The LFA-1 and Mac-1 antigens are composed of two chains or subunits, alpha and beta.
The beta chain of LFA-1 (Leukocyte Function Associated antigen) is immunologically identical to the beta chain of the Mac-1 antigen.
The line was recloned by TA Springer in 1988, and a new seed stock was created at the ATCC.
Complete Growth Medium
The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium:
  • fetal bovine serum to a final concentration of 10%
  • 0.05 mM 2-mercaptoethanol

Subculturing
Protocol: Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 5 X 10(4) viable cells/ml.
Interval: Maintain between 5 X 10(4) and 5 X 10(5) cells/ml.
Medium Renewal: Every 3 to 4 days
Cryopreservation
Freeze medium: Complete growth medium supplemented with 7.5% (v/v) DMSO
Storage temperature: liquid nitrogen vapor phase
Culture Conditions
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37.0°C
Isotype
rat IgG2a kappa
Name of Depositor
TA Springer
Deposited As
rat (B cell); mouse (myeloma)
References

Sanchez-Madrid F, et al. Mapping of antigenic and functional epitopes on the alpha-and beta-subunits of two related mouse glycoproteins involved in cell interactions, LFA-1 and MAC-1. J. Exp. Med. 158: 586-602, 1983. PubMed: 6193226

Sanchez-Madrid F, et al. Antigens involved in mouse cytolytic T-lymphocyte (CTL)-mediated killing: functional screening and topographic relationship. Cell. Immunol. 73: 1-11, 1982. PubMed: 6983917

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