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當(dāng)前位置: 首頁(yè) > ATCC代理 > M18/2.a.12.7 (new clone of M18/2.a.8) B165044
M18/2.a.12.7 (new clone of M18/2.a.8)
M18/2.a.12.7 (new clone of M18/2.a.8)
規(guī)格:
價(jià)格:
編號(hào):B165044
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱(chēng)
M18/2.a.12.7 (new clone of M18/2.a.8)
商品貨號(hào)
B165044
Organism
Rattus norvegicus (B cell); Mus musculus (myeloma), rat (B cell); mouse (myeloma)
Tissue
spleen
培養(yǎng)基
Cell Type
hybridoma: B lymphocyte
Product Format
frozen
Morphology
lymphoblast
Culture Properties
suspension
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Applications
The LFA-1 antigen is associated with antigen specific T lymphocyte mediated cell killing.
The LFA-1 and Mac-1 antigens are composed of two chains or subunits, alpha and beta.
Tested and found negative for ectromelia virus (mousepox).
Storage Conditions
liquid nitrogen vapor phase
Derivation
Spleen cells were fused with P3X63Ag8.653 myeloma cells.
The line was recloned by TA Springer in 1988, and a new seed stock was created at the ATCC.
Genes Expressed
immunoglobulin; monoclonal antibody; against the beta subunit of LFA-1 and Mac-1 (CD18)
Cellular Products
immunoglobulin; monoclonal antibody; against the beta subunit of LFA-1 and Mac-1 (CD18)
Comments
Animals were immunized with mouse cytotoxic T cell glycoproteins.
Spleen cells were fused with P3X63Ag8.653 myeloma cells.
The Mac-1 antigen is a macrophage differentiation antigen associated with type three complement receptor (CR3).
The LFA-1 antigen is associated with antigen specific T lymphocyte mediated cell killing.
The LFA-1 and Mac-1 antigens are composed of two chains or subunits, alpha and beta.
The beta chain of LFA-1 (Leukocyte Function Associated antigen) is immunologically identical to the beta chain of the Mac-1 antigen.
Tested and found negative for ectromelia virus (mousepox).
The line was recloned by TA Springer in 1988, and a new seed stock was created at the ATCC.
Complete Growth Medium
The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium:
  • fetal bovine serum to a final concentration of 10%
  • 0.05 mM 2-mercaptoethanol

The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium:
  • fetal bovine serum to a final concentration of 10%
  • 0.05 mM 2-mercaptoethanol

Subculturing
Protocol: Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 1 to 2 X 10(5) viable cells/ml.
Interval: Maintain cell density between 1 X 10(5) and 1 X 10(6) viable cells/ml.
Medium Renewal: Every 2 to 3 days
Cryopreservation
Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO
Storage temperature: liquid nitrogen vapor phase
Culture Conditions
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37.0°C
Isotype
rat IgG2a kappa
Name of Depositor
TA Springer
Deposited As
rat (B cell); mouse (myeloma)
References

Sanchez-Madrid F, et al. Mapping of antigenic and functional epitopes on the alpha-and beta-subunits of two related mouse glycoproteins involved in cell interactions, LFA-1 and MAC-1. J. Exp. Med. 158: 586-602, 1983. PubMed: 6193226

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