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M5/49.4.1

規格:

價格:

編號:B165056

品牌:Mingzhoubio

標準菌株

定量菌液

DNA

RNA

規格:

凍干粉

斜面

甘油

平板

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產品名稱

M5/49.4.1

商品貨號

B165056

Organism

Rattus norvegicus (B cell); Mus musculus (myeloma), rat (B cell); mouse (myeloma)

Tissue

spleen

培養基

Cell Type

hybridoma: B lymphocyte

Product Format

frozen

Morphology

lymphoblast

Culture Properties

suspension

Biosafety Level

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Disease

Lymphoma

Storage Conditions

liquid nitrogen vapor phase

Derivation

Spleen cells were fused with NS-1 myeloma cells.

Genes Expressed

immunoglobulin; monoclonal antibody; against Thy-1

Cellular Products

immunoglobulin; monoclonal antibody; against Thy-1

Comments

Animals were immunized with a secondary mixed lymphocyte culture (C57BL/6 mouse anti irradiated BN rat lymphoma).

Spleen cells were fused with NS-1 myeloma cells.

The antibody does not block CTL mediated cytotoxicity.

Complete Growth Medium

The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.

Subculturing

Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 1 X 10⁵ viable cells/mL.

Interval: Maintain cell density between 5 X 10⁴ and 5 X 10⁵ viable cells/mL.

Medium Renewal: Every 2 to 3 days

Cryopreservation

Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO

Storage temperature: liquid nitrogen vapor phase

Culture Conditions

Atmosphere: air, 95%; carbon dioxide (CO₂), 5%

Temperature: 37°C

Isotype

rat IgG2a

Name of Depositor

TA Springer

Deposited As

rat (B cell); mouse (myeloma)

References

Davignon D, et al. Lymphocyte function-associated antigen 1 (LFA-1): a surface antigen distinct from Lyt-2,3 that participates in T lymphocyte-mediated killing. Proc. Natl. Acad. Sci. USA 78: 4535-4539, 1981. PubMed: 7027264

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