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mAb 13-1

規格:

價格:

編號:B165063

品牌:Mingzhoubio

標準菌株

定量菌液

DNA

RNA

規格:

凍干粉

斜面

甘油

平板

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產品名稱

mAb 13-1

商品貨號

B165063

Organism

Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)

Cell Type

hybridoma: B lymphocyte

培養基

Product Format

frozen

Morphology

lymphoblast

Culture Properties

suspension

Biosafety Level

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Strain

Strain: BALB/c (B cell); unknown (myeloma)

Applications

The antibody binds an epitope comprising amino acids 729-736 of human CFTR.

The antibody can be used to purify CFTR from an impure solution containing CFTR, to detect CFTR in a biological sample, and to determine the orientation of CFTR in the membrane of a lipid vesicle.

Disclosure

This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC.

Derivation

Spleen cells were fused with mouse myeloma cells.

Genes Expressed

immunoglobulin; monoclonal antibody; against the cystic fibrosis transmembrane conductance regulator (CFTR)

Cellular Products

immunoglobulin; monoclonal antibody; against the cystic fibrosis transmembrane conductance regulator (CFTR)

Comments

Animals were immunized with a fusion protein comprising amino acids 591-829 (from exon 13) of the human cystic fibrosis transmembrane conductance regulator (CFTR) and beta galactosidase.

Spleen cells were fused with mouse myeloma cells.

The antibody binds an epitope comprising amino acids 729-736 of human CFTR.

The antibody can be used to purify CFTR from an impure solution containing CFTR, to detect CFTR in a biological sample, and to determine the orientation of CFTR in the membrane of a lipid vesicle.

Complete Growth Medium

The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.

Subculturing

Medium Renewal: Add fresh medium every 2 to 3 days (depending on cell density)

Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 2 X 10 exp5 viable cells/ml.

Maintain cell density between 2 X 10 exp5 and 1 X 10 exp6 viable cells/ml.

Cryopreservation

Culture medium 90%; DMSO, 10%

Culture Conditions

Temperature: 37.0°C

Isotype

IgG1

Name of Depositor

Genzyme Corp.

Deposited As

mouse (B cell); mouse (myeloma)

U.S. Patent Number