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Mab 2B5
Mab 2B5
規格:
價格:
編號:B165067
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱
Mab 2B5
商品貨號
B165067
Organism
Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)
Cell Type
hybridoma: B lymphocyte
培養基
Product Format
frozen
Morphology
lymphoblast
Culture Properties
suspension
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Strain

Strain: BALB/c (B cell); BALB/c (myeloma)

Disclosure
This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC.
Derivation
Animals were immunized with purified human lipopolysaccharide (LPS) binding protein (LBP)
Spleen cells were fused with P3X63Ag8.653 mouse myeloma cells.
Genes Expressed
immunoglobulin; monoclonal antibody; against human lipopolysaccharide (LPS) binding protein (LBP)
Cellular Products
immunoglobulin; monoclonal antibody; against human lipopolysaccharide (LPS) binding protein (LBP)
Comments
Animals were immunized with purified human lipopolysaccharide (LPS) binding protein (LBP)
The monoclonal antibody Mab 2B5 (ATCC HB-11491) recognizes overlapping epitopes on LPB whereas the monoclonal antibody Mab 1E8 (ATCC HB-11490) recognizes unique LBP epitopes.
Spleen cells were fused with P3X63Ag8.653 mouse myeloma cells.
Complete Growth Medium
The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing
Medium Renewal: Every 2 to 3 days
Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 1 X 10 exp5 viable cells/ml.
Maintain cell density between 1 X 10 exp5 and 1 X 10 exp6 viable cells/ml.
Cryopreservation
culture medium 95%; DMSO, 5%
Culture Conditions
Temperature: 37.0°C
Isotype
IgG1; kappa light chain
Name of Depositor
Scripps Research Inst.
Deposited As
mouse (B cell); mouse (myeloma)
U.S. Patent Number
References

Kirkland T, et al. Monoclonal antibodies immunoreactive with lipopolysaccharide binding protein (LBP) and methods of their use. US Patent 6,232,080 dated May 15 2001

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