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MBL-Sm-4B1
MBL-Sm-4B1
規格:
價格:
編號:B165095
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱
MBL-Sm-4B1
商品貨號
B165095
Organism
Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)
Cell Type
hybridoma: B lymphocyte
培養基
Product Format
frozen
Morphology
lymphoblast
Culture Properties
suspension
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Applications
The antibody is specific for one of the two major epitopes on S. mansoni paramyosin that predominate in the in vivo immune response to the parasite.
Tested and found negative for ectromelia virus (mousepox).
Derivation
Animals were immunized with a partially purified S. mansoni antigen preparation plus BCG.
Spleen cells were fused with P3X63Ag8.653 myeloma cells.
Genes Expressed
immunoglobulin; monoclonal antibody; against paramyosin from Schistosoma mansoni
Cellular Products
immunoglobulin; monoclonal antibody; against paramyosin from Schistosoma mansoni
Comments
Animals were immunized with a partially purified S. mansoni antigen preparation plus BCG.
Spleen cells were fused with P3X63Ag8.653 myeloma cells.
The antibody is specific for one of the two major epitopes on S. mansoni paramyosin that predominate in the in vivo immune response to the parasite. The epitope recognized is distinct from that recognized by MBL-Sm-1A6 (ATCC HB-194).
Tested and found negative for ectromelia virus (mousepox).
Complete Growth Medium
Culture Medium: Dulbecco's modified Eagle's medium with 4.5 g/L glucose, 90%;
fetal bovine serum, 10%.
Subculturing
Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
Isotype
IgG2a
Name of Depositor
A Sher, EJ Pearce
Deposited As
mouse (B cell); mouse (myeloma)
References

Lanar DE, et al. Identification of paramyosin as schistosome antigen recognized by intradermally vaccinated mice. Science 234: 593-596, 1986. PubMed: 3094144

Pearce EJ, et al. Immunochemical characterization and purification of Sm-97, a Schistosoma mansoni antigen monospecifically recognized by antibodies from mice protectively immunized with a nonliving vaccine. J. Immunol. 137: 3593-3600, 1986. PubMed: 2431037

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