Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
The cell line has been used extensively since 1976 as an H-2b cell line for cell-mediated cytotoxicity assays; the cells express both H-2Kb and H-2Db.
The cells express both H-2Kb and H-2Db.
MC57G is infectible by viruses.
Note: For more information on enzymatic dissociation and subculturing of cell lines consult Chapter 12 in Culture of Animal Cells: A Manual of Basic Technique by R. Ian Freshney, 4th edition, published by Wiley - Liss, N.Y., 2000.
Aden DP, Knowles BB. Cell surface antigens coded by the human chromosome 7. Immunogenetics 3: 209-221, 1976.
Trinchieri G, et al. Cell-mediated cytotoxicity to SV40-specific tumour-associated antigens. Nature 261: 312-314, 1976. PubMed: 179019
Doherty PC, et al. H-2 gene expression in required for T cell-mediated lysis of virus-infected target cells. Nature 266: 361-362, 1977. PubMed: 300845
Doherty PC, et al. Cytotoxic T-cell responses in mice infected with influenza and vaccinia viruses vary in magnitude with H-2 genotype. J. Exp. Med. 148: 534-543, 1978. PubMed: 100569
Wiktor TJ, et al. In vitro evidence of cell-mediated immunity after exposure of mice to both live and inactivated rabies virus. Proc. Natl. Acad. Sci. USA 74: 334-338, 1977. PubMed: 299948
This cell line was established from a tumor which developed after treatment with methylcholanthrene.
Hay, R. J., Caputo, J. L., and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC.
Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988.
Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC.
