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McA-RH8994
McA-RH8994
規格:
價格:
編號:B165105
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱
McA-RH8994
商品貨號
B165105
Organism
Rattus norvegicus, rat
Tissue
liver
培養基
Product Format
frozen
Morphology
epithelial
Culture Properties
adherent
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Disease
hepatoma; Morris hepatoma 8994
Gender
male
Storage Conditions
liquid nitrogen vapor phase
Clinical Data
male
Receptor Expression
glucocorticoid
Genes Expressed
alpha-fetoprotein (AFP)
Cellular Products
alpha-fetoprotein (AFP); alpha fetoprotein
Comments
Addition of glucocorticoids induces alpha fetoprotein synthesis and slows cell proliferation.

AFP is also synthesized when cell growth slows as the cells end the log phase of growth.
Complete Growth Medium
Dulbecco's modified Eagle's medium with 4 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate and 4.5 g/L glucose, 75%; horse serum, 20%; fetal bovine serum, 5%.
Subculturing
Remove medium, add fresh 0.25% trypsin solution, remove trypsin immediately and let the culture sit at room temperature for 5 to 10 minutes. Add fresh medium, aspirate and dispense into new flasks. Subculture once per week.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended
Medium Renewal: Every 2 or 3 days
Name of Depositor
JE Becker
Deposited As
Rattus sp.
References

Morris H, Meranze D. Induction and Some Characteristics of “Minimal Deviation” and Other Transplantable Rat Hepatomas. Recent Results Cancer Res. 44: 103-114, 1974.

Becker JE, et al. Two new rat hepatoma cell lines for studying the unbalanced blocked ontogeny hypothesisIn: Becker JE, et al. Onco-developmental gene expression. New YorkAcademic Press, pp. 259-270, 1976

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