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MD2
MD2
規格:
價格:
編號:B165117
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱
MD2
商品貨號
B165117
Organism
Rattus norvegicus (B cell); Mus musculus (myeloma), rat (B cell); mouse (myeloma)
Cell Type
hybridoma: B lymphocyte
培養基
Product Format
frozen
Morphology
lymphoblast
Culture Properties
suspension
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Applications
In the mouse thymus, MD2 is specific for dendritic cells in the medulla.
The antibody cross-reacts with rare cells in the human thymic medulla and faintly with Hassall's bodies.
It also reacts with epithelial elements in a variety of mouse tissues.
Derivation
Spleen cells were fused with Sp2/0 myeloma cells.
Genes Expressed
immunoglobulin; monoclonal antibody; against mouse medullary thymic epithelium
Cellular Products
immunoglobulin; monoclonal antibody; against mouse medullary thymic epithelium
Comments
Animals were immunized with mouse thymic stromal tissue.
Spleen cells were fused with Sp2/0 myeloma cells.
In the mouse thymus, MD2 is specific for dendritic cells in the medulla.
The antibody cross-reacts with rare cells in the human thymic medulla and faintly with Hassall's bodies.
It also reacts with epithelial elements in a variety of mouse tissues.
Complete Growth Medium
Culture Medium: RPMI medium 1640 with non-essential amino acids, 1 mM sodium
pyruvate and 50 uM 2-mercaptoethanol, 90%; fetal bovine serum, 10%.
Subculturing
Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
Isotype
IgM
Name of Depositor
RV Rouse
Deposited As
rat (B cell); mouse (myeloma)
References

Rouse RV, et al. Monoclonal antibodies reactive with subsets of mouse and human thymic epithelial cells. J. Histochem. Cytochem. 36: 1511-1517, 1988. PubMed: 2461413

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