Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
This line is hyperdiploid and there is a bi-modal chromosome number distribution. There are no consistent identifiable marker chromosomes. One normal X chromosome is present in most spreads.
The cells are positive for keratin by immunoperoxidase staining. MDCK cells have been used to study processing of beta amyloid precursor protein and sorting of its proteolytic products.
Didier ES, et al. Characterization of Encephalitozoon (Septata) intestinailis isolates cultured from nasal mucosa and bronchoalveolar lavage fluids of two AIDS patients. J. Eukaryot. Microbiol. 43: 34-43, 1996. PubMed: 8563708
Haass C, et al. Polarized sorting of beta-amyloid precursor protein and its proteolytic products in MDCK cells is regulated by two independent signals. J. Cell Biol. 128: 537-547, 1995. PubMed: 7860629
Gaush CR, et al. Characterization of an established line of canine kidney cells (MDCK). Proc. Soc. Exp. Biol. Med. 122: 931-935, 1966. PubMed: 5918973
Loffler S, et al. CD9, a tetraspan transmembrane protein, renders cells susceptible to canine distemper virus. J. Virol. 71: 42-49, 1997. PubMed: 8985321
Mead JR, et al. In vitro expression of mRNA coding for a Cryptosporidium parvum oocyst wall protein. J. Eukaryot. Microbiol. 43: 84-85, 1996. PubMed: 8822876
von Dippe P, et al. The functional expression of sodium-dependent bile acid transport in Madin-Darby canine kidney cells transfected with the cDNA for microsomal epoxide hydrolase. J. Biol. Chem. 271: 18176-18180, 1996. PubMed: 8663355
Panneerselvam K, Freeze HH. Mannose enters mammalian cells using a specific transporter that is insensitive to glucose. J. Biol. Chem. 271: 9417-9421, 1996. PubMed: 8621609
Stuart RO, et al. Dependence of epithelial intercellular junction biogenesis on thapsigargin-sensitive intracellular calcium stores. J. Biol. Chem. 271: 13636-13641, 1996. PubMed: 8662885
Grindstaff KK, et al. Translational regulation of Na,K-ATPase alpha1 and beta1 polypeptide expression in epithelial cells. J. Biol. Chem. 271: 23211-23221, 1996. PubMed: 8798517
Nucleotide (GenBank) : AB032025 Canis familiaris mRNA for ubiquitin, partial cds.
Nucleotide (GenBank) : D01020 Canis familiaris interleukin-8 gene, partial sequence.
