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MDTC-RP19

規格:

價格:

編號:B165133

品牌:Mingzhoubio

標準菌株

定量菌液

DNA

RNA

規格:

凍干粉

斜面

甘油

平板

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產品名稱

MDTC-RP19

商品貨號

B165133

Organism

Meleagris gallopavo, turkey

Tissue

lymph node

培養基

Product Format

frozen

Morphology

lymphoblast

Culture Properties

suspension

Biosafety Level

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Disease

Marek's disease

Disclosure

This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC.

Comments

The line is free of major oncogenic avian viruses.

The cells are pathogenic in chickens but fluids are not.

The cells grow well at 41C.

Complete Growth Medium

A 1:1 mixture of Leibovitz's L-15 medium and McCoy's 5a medium with 2 mM L-glutamine, 55%; tryptose phosphate broth, 5%; chicken serum, 20%; fetal bovine serum, 20% The fetal bovine serum concentration may be reduced to 10% after the cells have been in culture for 10 to 14 days.

Subculturing

Medium Renewal: Every 2 to 3 days

Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 5 X 10 exp5 viable cells/ml.

Maintain cultures at cell concentrations between 5 X 10 exp5 and 2 X 10 exp6 viable cells/ml.

Cryopreservation

Culture medium, 90%; DMSO, 10%

Culture Conditions

Temperature: 41.0°C

Atmosphere: air, 95%; carbon dioxide (CO₂), 5%

Name of Depositor

K Nazerian

Deposited As

Meleagris gallopavo

U.S. Patent Number

4,388,298

References

Nazerian K, et al. Establishment of B-lymphoblastoid cell lines from Marek's disease virus- induced tumors in turkeys. Int. J. Cancer 29: 63-68, 1982. PubMed: 6277804

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