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ME 1
ME 1
規格:
價格:
編號:B165134
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱
ME 1
商品貨號
B165134
Organism
Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)
Cell Type
hybridoma: B lymphocyte
培養基
Product Format
frozen
Morphology
lymphoblast
Culture Properties
suspension
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Applications
Animals were immunized with the JY human B lymphoblastoid cell line (HLA phenotype A11,A24,B7,B27) and with solubilized HLA A11, A24,B7,B27.
The antibody cross-reacts with HLA B14 and HLA Bw46.
Tested and found negative for ectromelia virus (mousepox).
Derivation
Spleen cells were fused with NS-1 myeloma cells.
Genes Expressed
immunoglobulin; monoclonal antibody; against HLA B7, Bw22, B27
Cellular Products
immunoglobulin; monoclonal antibody; against HLA B7, Bw22, B27
Comments
Animals were immunized with the JY human B lymphoblastoid cell line (HLA phenotype A11,A24,B7,B27) and with solubilized HLA A11, A24,B7,B27.
Spleen cells were fused with NS-1 myeloma cells.
The antibody cross-reacts with HLA B14 and HLA Bw46.
Tested and found negative for ectromelia virus (mousepox).
Complete Growth Medium
Modified Dulbecco's medium with 0.1 mM hypoxanthine and 0.016 mM thymidine 90%; fetal bovine serum, 10%
Subculturing
Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
Cryopreservation
Freeze medium: complete growth medium, 95%; DMSO, 5%.
Isotype
IgG1
Name of Depositor
P Parham
Deposited As
mouse (B cell); mouse (myeloma)
References

Ellis SA, et al. Recognition of HLA-B27 and related antigen by a monoclonal antibody. Hum. Immunol. 5: 49-59, 1982. PubMed: 6981636

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