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當(dāng)前位置: 首頁(yè) > ATCC代理 > ME-180 B165137
ME-180
ME-180
規(guī)格:
價(jià)格:
編號(hào):B165137
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱(chēng)
ME-180
商品貨號(hào)
B165137
Organism
Homo sapiens, human
Tissue
cervix; derived from metastatic site: omentum
培養(yǎng)基
Product Format
frozen
Morphology
epithelial
Culture Properties
adherent
Biosafety Level
2 [Cells contain human papilloma virus]

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Disease
epidermoid carcinoma
Age
66 years
Gender
female
Ethnicity
Caucasian
Storage Conditions
liquid nitrogen vapor phase
Karyotype
hyperdiploid to hypohexaploid with abnormalities including dicentrics, fragmentation and markers; unstable; (P63) hypotriploid with abnormalities including dicentrics and acrocentric fragments, large submetacentric and large subtelocentric markers
Derivation
This line was derived from a highly invasive squamous cell carcinoma with irregular cell clusters and no significant keratinization.
Clinical Data
66 years
Caucasian
female
Antigen Expression
Blood Type A; Rh+; HLA A1, A11, B5(+/-), B40
Oncogene
p53 +; pRB +
Genes Expressed
Oncogenes: p53 +; pRB +
Tumorigenic
Yes
Effects
Yes, in nude mice; forms well differentiated epidermoid carcinoma (grade I)
Comments
Desmosomal attachments were observed between cells in monolayer culture and cytoplasmic tonofilaments were noted.
Contamination with Mycoplasma arginini was detected and eliminated in 1970.
Growth of ME-180 cells is inhibited by tumor necrosis factor alpha (TNF alpha).
The cells contain human papillomavirus (HPV) DNA with greater homology to HPV-68 than HPV-18.
Complete Growth Medium
The base medium for this cell line is ATCC-formulated McCoy's 5a Medium Modified, Catalog No. 30-2007. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing
Remove medium, and rinse with 0.25% trypsin, 0.03% EDTA solution. Remove the solution and add an additional 1 to 2 mL of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37°C) until the cells detach. Add fresh culture medium, aspirate and dispense into new culture flasks.
Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:8 is recommended
Medium Renewal: 2 to 3 times per week
Cryopreservation
Freeze medium: Complete growth medium, 95%; DMSO, 5%
Storage temperature: liquid nitrogen vapor phase
Culture Conditions
Temperature: 37.0°C
STR Profile
Amelogenin: X
CSF1PO: 11
D13S317: 11,13
D16S539: 12,13
D5S818: 12
D7S820: 9,10
THO1: 8,9.3
TPOX: 8,10
vWA: 15,17
Isoenzymes
AK-1, 1
ES-D, 1-2
G6PD, B
GLO-I, 2
PGM1, 1-2
PGM3, 1
Name of Depositor
JA Sykes
Deposited As
Homo sapiens
U.S. Patent Number
5,981,173
References

Sugarman BJ, et al. Recombinant human tumor necrosis factor-alpha: effects on proliferation of normal and transformed cells in vitro. Science 230: 943-945, 1985. PubMed: 3933111

Sykes JA, et al. Some properties of a new epithelial cell line of human origin. J. Natl. Cancer Inst. 45: 107-122, 1970. PubMed: 4317734

Goodfellow M, et al. One hundred and twenty-seven cultured human tumor cell lines producing tumors in nude mice. J. Natl. Cancer Inst. 59: 221-226, 1977. PubMed: 77210034

Pater MM, Pater A. Human papillomavirus types 16 and 18 sequences in carcinoma cell lines of the cervix. Virology 145: 313-318, 1985. PubMed: 2992153

Yee C, et al. Presence and expression of human papillomavirus sequences in human cervical carcinoma cell lines. Am. J. Pathol. 119: 361-366, 1985. PubMed: 2990217

Pollack MS, et al. HLA-A, B, C and DR alloantigen expression on forty-six cultured human tumor cell lines. J. Natl. Cancer Inst. 66: 1003-1012, 1981. PubMed: 7017212

Reuter S, et al. Characterization of a novel human papillomavirus DNA in the cervical carcinoma cell line ME180. J. Virol. 65: 5564-5568, 1991. PubMed: 1716694

Scheffner M, et al. The state of the p53 and retinoblastoma genes in human cervical carcinoma cell lines. Proc. Natl. Acad. Sci. USA 88: 5523-5527, 1991. PubMed: 1648218

Hendricks DT, et al. FHIT gene expression in human ovarian, endometrial, and cervical cancer cell lines. Cancer Res. 57: 2112-2115, 1997. PubMed: 9187105

van Dijk MA, et al. A functional assay in yeas for the human estrogen receptor displays wild-type and variant estrogen receptor messenger RNAs present in breast carcinoma. Cancer Res. 57: 3478-3485, 1997. PubMed: 9270016

St. Geme JW, et al. Characterization of the genetic locus encoding Haemophilus influenzae type b surface fibrils. J. Bacteriol. 178: 6281-6287, 1996. PubMed: 8892830

Longuet M, Beaudenon S, Orth G. Two novel genital human papillomavirus (HPV) types, HPV68 and HPV70, related to the potentially oncogenic HPV39. J Clin Microbiol 34(3): 738-744, 1996. PubMed: 8904450

Cellosaurus ME-180 (CVCL_1401).

U.S. Patent 5,981,173

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