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ME361-S2a [ME361S2a]
ME361-S2a [ME361S2a]
規格:
價格:
編號:B165138
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱
ME361-S2a [ME361S2a]
商品貨號
B165138
Organism
Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)
Cell Type
hybridoma: B lymphocyte
培養基
Product Format
frozen
Morphology
lymphoblast
Culture Properties
suspension
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Strain

Strain:? BALB/c (B cell); BALB/c (myeloma)

Applications
The antibody reacts with GD2 and GD3 gangliosides.
It reacts preferentially with the GD2 and is largely unreactive with other gangliosides.
It may be useful for diagnosis and therapy of human melanoma.
Disclosure
This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC.
Derivation
Spleen cells were fused with P3X63Ag8.653 myeloma cells.
Clinical Data
It may be useful for diagnosis and therapy of human melanoma.
Genes Expressed
immunoglobulin; monoclonal antibody; against GD2 and GD3 gangliosides
Cellular Products
immunoglobulin; monoclonal antibody; against GD2 and GD3 gangliosides
Comments
Animals were immunized with the SK MEL 23 human melanoma cells line.
Spleen cells were fused with P3X63Ag8.653 myeloma cells.
The antibody reacts with GD2 and GD3 gangliosides.
It reacts preferentially with the GD2 and is largely unreactive with other gangliosides.
It may be useful for diagnosis and therapy of human melanoma.
This line is an isotype switch variant of ME361 (see ATCC HB-9325).
Complete Growth Medium
The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing
Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
Cryopreservation
culture medium, 85%; fetal bovine serum, 10%; DMSO, 5%
Culture Conditions
Temperature: 37.0°C
Isotype
IgG2a
Name of Depositor
Wistar Institute
Deposited As
mouse (B cell); mouse (myeloma)
U.S. Patent Number
References

Thurin J, et al. Monoclonal antibodies against melanoma-associated antigens and hybrid cell lines producing these antibodies. US Patent 4,849,509 dated Jul 18 1989

Thurin J, et al. GD2 ganglioside biosynthesis is a distinct biochemical event in human melanoma tumor progression. FEBS Lett. 208: 17-22, 1986. PubMed: 3533633

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