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MECA-367
MECA-367
規格:
價格:
編號:B165139
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱
MECA-367
商品貨號
B165139
Organism
Rattus norvegicus (B cell); Mus musculus (myeloma), rat (B cell); mouse (myeloma)
Cell Type
hybridoma: B lymphocyte
培養基
Product Format
frozen
Morphology
lymphoblast
Culture Properties
suspension
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Applications
The antibody specifically blocks lymphocyte adhesion to mouse Peyer's patch endothelia and does not block lymphocyte binding to peripheral lymph nodes.
Disclosure
This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC.
Derivation
Spleen cells were fused with Sp2/0-Ag14 myeloma cells.
Genes Expressed
immunoglobulin; monoclonal antibody; against mouse Peyer's patch endothelial cells (MAdCAM-1)
Cellular Products
immunoglobulin; monoclonal antibody; against mouse Peyer's patch endothelial cells (MAdCAM-1)
Comments
Animals were immunized with mouse lymphnode stroma.
Spleen cells were fused with Sp2/0-Ag14 myeloma cells.
The antibody specifically blocks lymphocyte adhesion to mouse Peyer's patch endothelia and does not block lymphocyte binding to peripheral lymph nodes.
It recognizes the N terminal domain of mouse mucosal vascular addressin (MAdCAM-1) involved in alpha 4, beta 7 (a4b7) integrin binding.
Complete Growth Medium
Culture Medium: RPMI 1640 medium, 90%; fetal bovine serum, 10%.
Subculturing
Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
Culture Conditions
Temperature: 37.0°C
Isotype
IgG2a
Name of Depositor
Stanford University Board of Trustees
U.S. Patent Number
References

Butcher EC. Method to control leukocyte extravasation. US Patent 5,403,919 dated Apr 4 1995

Streeter PR, et al. A tissue-specific endothelial cell molecule involved in lymphocyte homing. Nature 331: 41-46, 1988. PubMed: 3340147

Berlin C, et al. Alpha 4 beta 7 integrin mediates lymphocyte binding to the mucosal vascular addressin MAdCAM-1. Cell 74: 185-195, 1993. PubMed: 7687523

Briskin MJ, et al. MAdCAM-1 has homology to immunoglobulin and mucin-like adhesion receptors and to IgA1. Nature 363: 461-464, 1993. PubMed: 8502297

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