最新中文字幕-精品国产99-国产精品福利在线观看-粉嫩av一区二区三区-日韩精品久久久久-美丽的姑娘国语视频-亚洲欧洲色-国产不卡毛片-三级黄毛片-免费福利在线-日韩av三区-欧美成年人网站-欧洲美女与动交ccoo-成人涩涩软件-秋霞影院午夜老牛影院-婷婷久久伊人-呦呦视频在线观看-www.天天综合-日韩精品高清视频-国产欧美综合在线-欧美日韩一区二区三区在线视频-a猛片免费播放-91九色蝌蚪国产-国产欧美日韩专区-嫩模被强到高潮呻吟不断

歷史搜索:A549    293T 金黃色葡萄球菌 大腸桿菌 AKK菌
購物車 1 種商品 - 共0元
當前位置: 首頁 > ATCC代理 > MEL-14 [MEL-14.D54] B165144
MEL-14 [MEL-14.D54]
MEL-14 [MEL-14.D54]
規格:
價格:
編號:B165144
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱
MEL-14 [MEL-14.D54]
商品貨號
B165144
Organism
Rattus norvegicus (B cell); Mus musculus (myeloma), rat (B cell); mouse (myeloma)
Cell Type
hybridoma: B lymphocyte
培養基
Product Format
frozen
Morphology
lymphoblast
Culture Properties
suspension
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Disease
Lymphoma
Applications
Animals were immunized with 38C-13 cells (a B cell lymphoma cell line derived from a C3H/eb mouse).
The antibody is specific for a 90000 dalton lymphocyte surface receptor for high endothelial venule determinants in peripheral lymph nodes.
The antibody binds to a to a determinant present in a peptide containing the 13 C terminal residues of ubiquitin.
Tested and found negative for ectromelia virus (mousepox).
Derivation
Animals were immunized with 38C-13 cells (a B cell lymphoma cell line derived from a C3H/eb mouse).
Spleen cells were fused with P3X63Ag8.653 myeloma cells.
Genes Expressed
immunoglobulin; monoclonal antibody; against lymphocyte surface receptor for endothelium (murine equivalent to human L-selectin, CD62L, Leu-8)
Cellular Products
immunoglobulin; monoclonal antibody; against lymphocyte surface receptor for endothelium (murine equivalent to human L-selectin, CD62L, Leu-8)
Comments
Animals were immunized with 38C-13 cells (a B cell lymphoma cell line derived from a C3H/eb mouse).
Spleen cells were fused with P3X63Ag8.653 myeloma cells.
The antibody is specific for a 90000 dalton lymphocyte surface receptor for high endothelial venule determinants in peripheral lymph nodes. This receptor appears to be required for lymphocyte homing into lymph nodes in vivo.
The antibody binds to a to a determinant present in a peptide containing the 13 C terminal residues of ubiquitin.
It does not bind to native ubiquitin or to ubiquitinated proteins.
Tested and found negative for ectromelia virus (mousepox).
Complete Growth Medium
The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing
Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
Cryopreservation
Freeze medium: complete growth medium, 95%; DMSO, 5%
Isotype
IgG2a
Name of Depositor
EC Butcher
Deposited As
rat (B cell); mouse (myeloma)
References

Gallatin WM, et al. A cell-surface molecule involved in organ-specific homing of lymphocytes. Nature 304: 30-34, 1983. PubMed: 6866086

Siegelman M, et al. Cell surface molecule associated with lymphocyte homing is a ubiquitinated branched-chain glycoprotein. Science 231: 823-829, 1986. PubMed: 3003913

Wilson ME, et al. Local suppression of IFN-gamma in hepatic granulomas correlates with tissue-specific replication of Leishmania chagasi. J. Immunol. 156: 2231-2239, 1996. PubMed: 8690913

  • 王經理
    手機號：17280875617
    QQ：1438578920
  • 胡經理
    手機號：13345964880
    QQ：2438244627
  • 周經理
    手機號：17757487661
    QQ：1296385441
  • 于經理
    手機號：18067160830
    QQ：2088210172
  • 沈經理
    手機號：19548299266
    QQ：2662369050
  • 李經理
    手機號：13626845108
    QQ：972239479