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MG-63

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價格:

編號:B165149

品牌:Mingzhoubio

標準菌株

定量菌液

DNA

RNA

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凍干粉

斜面

甘油

平板

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產品名稱

MG-63

商品貨號

B165149

Organism

Homo sapiens, human

Tissue

bone

培養基

Product Format

frozen

Morphology

fibroblast

Culture Properties

adherent

Biosafety Level

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Disease

osteosarcoma

Age

14 years

Gender

male

Ethnicity

Caucasian

Applications

This cell line is a suitable transfection host.

Storage Conditions

liquid nitrogen vapor phase

Karyotype

This is a hypotriploid human cell line. The modal chromosome number was 66 occurring in 44% of cells. The rate of cells with higher ploidies was 2.0%. Eighteen to 19 marker chromosomes were common to all cells.

Clinical Data

14 years

Caucasian

male

Receptor Expression

transforming growth factor beta (TGF-beta) RI, expressed

transforming growth factor beta (TGF-beta) RII, expressed

Genes Expressed

interferon

Cellular Products

interferon

Comments

High levels of interferon production can be induced using polyinosinic - polycytidylic acid, cycloheximide and actinomycin D.

Complete Growth Medium

The base medium for this cell line is ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: heat-inactivated fetal bovine serum to a final concentration of 10%.

Subculturing

Volumes are given for a 75 cm² flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.

Remove and discard culture medium.
Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.
Add appropriate aliquots of the cell suspension to new culture vessels.
Incubate cultures at 37°C.

Subcultivation Ratio: A subcultivation ratio of 1:4 to 1:8 is recommended

Medium Renewal: 2 to 3 times per week

Cryopreservation

Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO

Storage temperature: liquid nitrogen vapor phase

Culture Conditions

Atmosphere: air, 95%; carbon dioxide (CO₂), 5%

Temperature: 37°C

STR Profile

Amelogenin: X,Y

CSF1PO: 10,12

D13S317: 11

D16S539: 11

D5S818: 11,12

D7S820: 10

THO1: 9.3

TPOX: 8,11

vWA: 16,19

Name of Depositor

A Billiau

Deposited As

Homo sapiens

References

Billiau A, et al. Human interferon: mass production in a newly established cell line, MG-63. Antimicrob. Agents Chemother. 12: 11-15, 1977. PubMed: 883813

Takeuchi Y, et al. Relationship between actions of transforming growth factor (TGF)-beta and cell surface expression of its receptors in clonal osteoblastic cells. J. Cell. Physiol. 162: 315-321, 1995. PubMed: 7860639

Yee A, et al. Biochemical characterization of the human cyclin-dependent protein kinase activating kinase. J. Biol. Chem. 271: 471-477, 1996. PubMed: 8550604

Cross References

Nucleotide (GenBank) : NM_002214 Homo sapiens integrin, beta 8 (ITGB8), mRNA

Nucleotide (GenBank) : M73780 Human integrin beta-8 subunit mRNA, complete cds.

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