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MH-SVM33C9

規(guī)格:

價(jià)格:

編號(hào):B165156

品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株

定量菌液

DNA

RNA

規(guī)格:

凍干粉

斜面

甘油

平板

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產(chǎn)品名稱

MH-SVM33C9

商品貨號(hào)

B165156

Organism

Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)

Cell Type

hybridoma: B lymphocyte

培養(yǎng)基

Product Format

frozen

Morphology

lymphoblast

Culture Properties

suspension

Biosafety Level

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Applications

The antibody reacts specifically with HIV-1 p17.

Disclosure

This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC.

Derivation

Animals were immunized with lysates of purified HIV-1.

Spleen cells were fused with NS-1 myeloma cells.

Genes Expressed

immunoglobulin; monoclonal antibody; against HTLV-III (HIV-1, human immunodeficiency virus - 1) p17 (17000 dalton protein)

Cellular Products

immunoglobulin; monoclonal antibody; against HTLV-III (HIV-1, human immunodeficiency virus - 1) p17 (17000 dalton protein)

Comments

Animals were immunized with lysates of purified HIV-1.

Spleen cells were fused with NS-1 myeloma cells.

The antibody reacts specifically with HIV-1 p17.

Complete Growth Medium

Dulbecco's modified Eagle's medium with 4.5 g/L glucose, 2 mM L-glutamine, and 1 mM sodium pyruvate, 80%; fetal bovine serum, 20%

Subculturing

Medium Renewal: Every 2 to 3 days

Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.

Isotype

IgG1; kappa light chain

Name of Depositor

Akzo Nobel N.V.

U.S. Patent Number

4,843,011

References

Sarngadharan MG, et al. Monoclonal antibodies for binding HTLV-III proteins, and cell lines for their production. US Patent 4,843,011 dated Jun 27 1989

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