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MLH1
MLH1
規(guī)格:
價(jià)格:
編號(hào):B165165
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱
MLH1
商品貨號(hào)
B165165
Organism
Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)
Cell Type
hybridoma: B lymphocyte
培養(yǎng)基
Product Format
frozen
Morphology
lymphoblast
Culture Properties
suspension
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Applications
The antibody reacts specifically with the 26000 dalton polypeptide of the chloroplast thylakoid.
This antibody and that of MLH2 (ATCC CRL-1779) may be useful in studies of the LHC-II gene family (Cab) and in studying the photoregulation of the Cab genes in chloroplast development.
Tested and found negative for ectromelia virus (mousepox).
Derivation
Animals were immunized with purified LHC-II.
Spleen cells were fused with Sp2/0-Ag14 myeloma cells.
Genes Expressed
immunoglobulin; monoclonal antibody; against the light harvesting chlorophyll a/b protein complex (LHC-II) of higher plants
Cellular Products
immunoglobulin; monoclonal antibody; against the light harvesting chlorophyll a/b protein complex (LHC-II) of higher plants
Tumorigenic
Yes
Effects
Yes, in BALB/c mice
Comments
Animals were immunized with purified LHC-II.
Spleen cells were fused with Sp2/0-Ag14 myeloma cells.
The antibody reacts specifically with the 26000 dalton polypeptide of the chloroplast thylakoid.
This antibody and that of MLH2 (ATCC CRL-1779) may be useful in studies of the LHC-II gene family (Cab) and in studying the photoregulation of the Cab genes in chloroplast development.
Tested and found negative for ectromelia virus (mousepox).
Complete Growth Medium
The base medium for this cell line is ATCC-formulated Iscove's Modified Dulbecco's Medium, Catalog No. 30-2005. To make the complete growth medium, add the following components to the base medium:
  • 0.05 mM 2-mercaptoethanol
  • fetal bovine serum to a final concentration of 10%

Subculturing
Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
Isotype
IgG1
Name of Depositor
SC Darr
Deposited As
mouse (B cell); mouse (myeloma)
References

Darr SC, et al. Monoclonal antibodies to the light-harvesting chlorophyll a/b protein complex of photosystem II. J. Cell Biol. 103: 733-740, 1986. PubMed: 3528171

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