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MLTC-1

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價格:

編號:B165167

品牌:Mingzhoubio

標準菌株

定量菌液

DNA

RNA

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凍干粉

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甘油

平板

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產品名稱

MLTC-1

商品貨號

B165167

Organism

Mus musculus, mouse

Tissue

testis

培養基

Cell Type

Leydig cell

Product Format

frozen

Morphology

epithelial

Culture Properties

adherent, Cells form islands that dome and the monolayer may not become confluent.

Biosafety Level

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Disease

Leydig cell tumor

Gender

male

Strain

C57BL/6

Applications

This cell line is a suitable transfection host.

Karyotype

polyploid; modal number = 95 Ref

Rebois RV. Establishment of gonadotropin-responsive murine leydig tumor cell line. J. Cell Biol. 94: 70-76, 1982. PubMed: 6288740

Derivation

This line was established from the M548OP transplantable Leydig cell tumor carried in C57BL/6 mice.Ref

Rebois RV. Establishment of gonadotropin-responsive murine leydig tumor cell line. J. Cell Biol. 94: 70-76, 1982. PubMed: 6288740

Clinical Data

male

Receptor Expression

chorionic gonadotropin; luteinizing hormone (LH)

Ref

Rebois RV. Establishment of gonadotropin-responsive murine leydig tumor cell line. J. Cell Biol. 94: 70-76, 1982. PubMed: 6288740

Genes Expressed

progesterone

Ref

Rebois RV. Establishment of gonadotropin-responsive murine leydig tumor cell line. J. Cell Biol. 94: 70-76, 1982. PubMed: 6288740

Cellular Products

progesterone

Ref

Rebois RV. Establishment of gonadotropin-responsive murine leydig tumor cell line. J. Cell Biol. 94: 70-76, 1982. PubMed: 6288740

Tumorigenic

Yes

Effects

Yes, in C57BL/6 mice

Comments

The cells retain the hormonal responsiveness of the original tumor.

Membrane adenyl cyclase activity is stimulated by treatment with human chorionic gonadotropin (hCG), luteinizing hormone, cholera toxin, sodium fluoride and guanyl-5'-ylimidodiphosphate, and produce progesterone in response to hCG. Ref

Rebois RV. Establishment of gonadotropin-responsive murine leydig tumor cell line. J. Cell Biol. 94: 70-76, 1982. PubMed: 6288740

Complete Growth Medium

The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, ATCC 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum (ATCC 30-2020) to a final concentration of 10%.

Subculturing

Remove medium, and rinse with 0.25% trypsin, 0.03% EDTA solution. Remove the solution and add an additional 1 to 2 mL of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37°C) until the cells detach.

Add fresh culture medium, aspirate and dispense into new culture flasks.

Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:6 is recommended

Medium Renewal: Every 3 to 4 days

Cryopreservation

Culture medium, 95%; DMSO, 5%

Culture Conditions

Temperature: 37°C

Population Doubling Time

35 to 40 hrs

Name of Depositor

RV Rebois

Deposited As

Mus musculus

References

Rebois RV. Establishment of gonadotropin-responsive murine leydig tumor cell line. J. Cell Biol. 94: 70-76, 1982. PubMed: 6288740

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