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MOP-8
MOP-8
規格:
價格:
編號:B165185
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱
MOP-8
商品貨號
B165185
Organism
Mus musculus, mouse
Tissue
embryo
培養基
Cell Type
SV40 transformed
Product Format
frozen
Morphology
fibroblast
Culture Properties
adherent
Biosafety Level
2 Cells contain polyomavirus DNA sequences

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Age
embryo
Strain
NIH/Swiss
Applications
transfection host
Transfection host, especially for plasmids containing the polyomavirus origin of replication.
Derivation
The MOP-8 cell line was derived by transforming NIH 3T3 cells with a hybrid transcription unit composed of the SV40 early promoter fused to the early region of polyomavirus.
Comments
The MOP-8 cell line was derived by transforming NIH 3T3 cells with a hybrid transcription unit composed of the SV40 early promoter fused to the early region of polyomavirus.
The cells will support the replication of recombinant plasmids which contain the polyomavirus origin for DNA synthesis.
Tested and found negative for ectromelia virus (mousepox).
Complete Growth Medium
The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:4 is recommended
Medium Renewal: 2 to 3 times per week
Remove medium, add fresh 0.25% trypsin solution for 2 to 3 minutes, remove trypsin and let the culture sit at room temperature for 10 to 15 minutes. Add fresh medium, aspirate and dispense into new flasks.
Name of Depositor
JA Hassell
Deposited As
Mus musculus
References

Muller WJ, et al. Isolation of large T antigen-producing mouse cell lines capable of supporting replication of polyomavirus-plasmid recombinants. Mol. Cell. Biol. 4: 2406-2412, 1984. PubMed: 6096696

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