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MSTO-211H
MSTO-211H
規格:
價格:
編號:B165198
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱
MSTO-211H
商品貨號
B165198
Organism
Homo sapiens, human
Tissue
lung; derived from metastatic site: pleural effusion
培養基
Product Format
frozen
Morphology
fibroblast
Culture Properties
adherent
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Disease
biphasic mesothelioma
Age
62 years
Gender
male
Ethnicity
Caucasian
Storage Conditions
liquid nitrogen vapor phase
Karyotype
modal number = 72; range = 70 to 78
Derivation
The MSTO-211H cell line was established in 1985 from the pleural effusion of a patient with biphasic mesothelioma of the lung.
Derived from metastatic site, lung
Clinical Data
The patient had not received prior radiation or chemotherapy.
62 years
Caucasian
male
The MSTO-211H cell line was established in 1985 from the pleural effusion of a patient with biphasic mesothelioma of the lung.
Oncogene
c-myc +; v-src +; v-abl +; v-erb B +; c-raf 1 +; H-ras +; K-ras +; N-ras +; N-myc -; L-myc -; c-myb -; c-fos -; v-fes -; v-fms -; v-sis -
Tumorigenic
Yes
Effects
Yes, tumors for med in approximately 20% of nude mice inoculated with MSTO-211H cells
Comments

The patient had not received prior radiation or chemotherapy.

MSTO-211H cells have high affinity binding sites for EGF, and express Neuron specific enolase (NSE) and both the alpha and beta subunits of human chorionic gonadotropin (HCG).

L-DOPA decarboxylase (DDC), bombesin and neurotensin were not detected.

The cells overexpress the c-myc protooncogene, and no gene rearrangement or amplification was observed.

They are positive for the expression of v-src, v-abl, v-erb B, c-raf 1, Ha-ras, Ki-ras, and N-ras.
Expression of N-myc, L-myc, c-myb, c-fos, v-fes, v-fms and v-sis oncogene expression was not detected.

The cells can reach a saturation density of 400000 cells per sq cm, but will slough off of the surface as they attain this density.




Complete Growth Medium
The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, ATCC 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum (ATCC 30-2020) to a final concentration of 10%.
Subculturing
Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.
  1. Remove and discard culture medium.
  2. Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum which contains trypsin inhibitor.
  3. Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
    Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
  4. Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.
  5. Add appropriate aliquots of the cell suspension to new culture vessels.
  6. Incubate cultures at 37°C.
Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:6 is recommended
Medium Renewal: Every 2 to 3 days
Cryopreservation
Freeze medium: Complete growth medium 95%; DMSO, 5%
Storage temperature: liquid nitrogen vapor phase
Culture Conditions
Temperature: 37°C
STR Profile
Amelogenin: X,Y
CSF1PO: 11,12
D13S317: 11,14
D16S539: 13
D5S818: 12
D7S820: 8,12
THO1: 8,9.3
TPOX: 11
vWA: 16,18
Population Doubling Time
20 hrs
Name of Depositor
G Bepler
Deposited As
Homo sapiens
Year of Origin
1985
References

Bepler G, Koehler A. Multiple chromosomal aberrations and 11p allelotyping in lung cancer cell lines. Cancer Genet. Cytogenet. 84: 39-45, 1995. PubMed: 7497441

Bepler G, et al. Characterization of the state of differentiation of six newly established human non-small-cell lung cancer cell lines. Differentiation 37: 158-171, 1988. PubMed: 2840315

Haeder M, et al. Epidermal growth factor receptor expression in human lung cancer cell lines. Cancer Res. 48: 1132-1136, 1988. PubMed: 2830015

Lung Cancer 4: 155-161, 1988.

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