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MY904 [904]
MY904 [904]
規格:
價格:
編號:B165208
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱
MY904 [904]
商品貨號
B165208
Organism
Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)
Cell Type
hybridoma: B lymphocyte
培養基
Product Format
frozen
Morphology
lymphoblast
Culture Properties
suspension
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Disease
Leukemia
Strain

Strain:? BALB/c (B cell); BALB/c (myeloma)

Applications
The antibody reacts with granulocytes, monocytes and a subset of large granular lymphocytes.
The antibody can be used to reduce the inflammatory response of leukocytes in vivo.
Disclosure
This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC.
Derivation
Spleen cells were fused with NS-1 myeloma cells.
Genes Expressed
immunoglobulin; monoclonal antibody; against human inflammatory cells (phagocytic leukocytes); against CD11b
Cellular Products
immunoglobulin; monoclonal antibody; against human inflammatory cells (phagocytic leukocytes); against CD11b
Comments
Animals were immunized with human chronic granulocytic leukemia (CGL) cells.
Spleen cells were fused with NS-1 myeloma cells.
The antibody reacts with granulocytes, monocytes and a subset of large granular lymphocytes.
It inhibits adhesion related functions, but does not block iC3b binding.
The antibody can be used to reduce the inflammatory response of leukocytes in vivo.
Complete Growth Medium
The base medium for this cell line is ATCC-formulated Iscove's Modified Dulbecco's Medium, Catalog No. 30-2005. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing
Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
Cryopreservation
culture medium 95%; DMSO, 5%
Culture Conditions
Temperature: 37.0°C
Isotype
IgG1
Name of Depositor
Univ. Michigan, Dana Farber Cancer Inst.
U.S. Patent Number
References

Lucchesi BR, et al. Method of reducing tissue damage at an inflammatory site using a monoclonal antibody. US Patent 4,840,793 dated Jun 20 1989

Dana N, et al. Two functional domains in the phagocyte membrane glycoprotein Mo1 identified with monoclonal antibodies. J. Immunol. 137: 3259-3263, 1986. PubMed: 2430017

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