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NBT-II [Nara Bladder Tumor No. 2]

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編號:B165226

品牌:Mingzhoubio

標準菌株

定量菌液

DNA

RNA

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凍干粉

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甘油

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產品名稱

NBT-II [Nara Bladder Tumor No. 2]

商品貨號

B165226

Organism

Rattus norvegicus, rat

Tissue

urinary bladder

培養基

Product Format

frozen

Morphology

epithelial

Culture Properties

adherent

Biosafety Level

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Disease

tumor

Gender

male

Strain

Wistar

Storage Conditions

liquid nitrogen vapor phase

Derivation

The line was derived from a tumor which was induced with N-butyl-N-(4-hydroxybutyl) nitrosamine.

Comments

NBT-II cells differentiate in vitro giving rise to tumor giant cells.

The differentiation is inhibited by vitamin A.

Complete Growth Medium

The base medium for this cell line is ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.

Subculturing

Volumes used in this protocol are for 75 cm² flask; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes.

Remove and discard culture medium.
Briefly rinse the cell layer with 0.25% (w/v) Trypsin-0.03% (w/v) EDTA solution (ATCC 30-2101) to remove all traces of serum which contains trypsin inhibitor.
Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.
Add appropriate aliquots of the cell suspension to new culture vessels.
Incubate cultures at 37°C.

Subcultivation Ratio: A subcultivation ratio of 1:4 to 1:10 is recommended
Medium Renewal: 2 to 3 times per week
Note: For more information on enzymatic dissociation and subculturing of cell lines consult Chapter 10 in Culture of Animal Cells, a manual of Basic Technique by R. Ian Freshney, 3rd edition, published by Alan R. Liss, N.Y., 1994

Cryopreservation

Complete growth medium 95%; DMSO, 5%

Culture Conditions

Temperature: 37°C

Name of Depositor

J Leighton, R Tchao

Deposited As

Rattus sp.

References

Toyoshima K, et al. Tissue culture of urinary bladder tumor induced in a rat by N-butyl-N-(- 4-hydroxybutyl)nitrosamine: establishment of cell line, Nara Bladder Tumor II. J. Natl. Cancer Inst. 47: 979-985, 1971. PubMed: 4941696

Geisinger KR, et al. Appearance of giant cells as a morphological response of rat bladder carcinoma cell line (Nara Bladder Tumor No. 2) to a continuous thermal gradient in tissue culture. Cancer Res. 38: 1223-1230, 1978. PubMed: 639055

Toyoshima K, Leighton J. Vitamin A inhibition of keratinization in rat urinary bladder cancer cell line Nara Bladder Tumor No. 2 in meniscus gradient culture. Cancer Res. 35: 1873-1879, 1975. PubMed: 1093679

Tchao R. Novel forms of epithelial cell motility on collagen and on glass surfaces. Cell Motil. 2: 333-341, 1982. PubMed: 7151140

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