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NCI-H1404 [H1404]
NCI-H1404 [H1404]
規格:
價格:
編號:B165262
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱
NCI-H1404 [H1404]
商品貨號
B165262
Organism
Homo sapiens, human
Tissue
lung
培養基
Product Format
frozen
Morphology
epithelial
Culture Properties
suspension, multicell aggregates
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Disease
papillary adenocarcinoma
Age
48 years
Gender
male
Ethnicity
Caucasian
Applications
This line was derived by A.F. Gazdar, H.K. Oie, J.D. Minna and associates from a lymph node metastasis obtained from a patient prior to therapy.
Ultrastructural studies demonstrated the presence of cytoplasmic structures characteristic of Clara cells.
Storage Conditions
liquid nitrogen vapor phase
Derivation
This line was derived by A.F. Gazdar, H.K. Oie, J.D. Minna and associates from a lymph node metastasis obtained from a patient prior to therapy.
Clinical Data
This line was derived by A.F. Gazdar, H.K. Oie, J.D. Minna and associates from a lymph node metastasis obtained from a patient prior to therapy.
male
Caucasian
48 years
Genes Expressed
surfactant associated protein A (SP-A); surfactant associated protein B (SP-B)
Cellular Products
surfactant associated protein A (SP-A); surfactant associated protein B (SP-B)
Tumorigenic
Yes
Effects
Yes, the cells produce tumors in athymic nude mice
Comments
This line was derived by A.F. Gazdar, H.K. Oie, J.D. Minna and associates from a lymph node metastasis obtained from a patient prior to therapy.
Ultrastructural studies demonstrated the presence of cytoplasmic structures characteristic of Clara cells.
The cells express protein and RNA of SP-A, the major surfactant associated protein, and SP-B.
SP-C RNA was not expressed.
Complete Growth Medium
The base medium for this cell line is RPMI-1640 Medium (ATCC 30-2001).To make the complete growth medium, add the following components to the base medium: Fetal Bovine Serum (FBS; ATCC 30-2020) to a final concentration of 5%.
Subculturing
Protocol: Cultures can be maintained by addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation of the suspension with subsequent resuspension in fresh medium. Add medium as the cell density increases.
Medium Renewal: Every 2 to 3 days
Cryopreservation
Freeze medium: Complete growth medium supplemented with 10% fetal bovine serum and 5% (v/v) DMSO
Storage temperature: liquid nitrogen vapor phase
Culture Conditions
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37.0°C
STR Profile
Amelogenin: X
CSF1PO: 10
D13S317: 9,14
D16S539: 12
D5S818: 12
D7S820: 11,14
THO1: 7,9.3
TPOX: 9,11
vWA: 19
Name of Depositor
AF Gazdar, JD Minna
Deposited As
Homo sapiens
References

Gazdar AF, et al. Peripheral airway cell differentiation in human lung cancer cell lines. Cancer Res. 50: 5481-5487, 1990. PubMed: 2386953

NCI-Navy Medical Oncology Branch Cell Line Supplement. J. Cell. Biochem. suppl. 24: 1996.

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