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NCI-H1819 [H1819]
NCI-H1819 [H1819]
規(guī)格:
價(jià)格:
編號:B165288
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱
NCI-H1819 [H1819]
商品貨號
B165288
Organism
Homo sapiens, human
Tissue
lung; derived from metastatic site: lymph node
培養(yǎng)基
Product Format
frozen
Culture Properties
mixed, adherent and suspension
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Disease
adenocarcinoma
Age
55 years
Gender
female
Ethnicity
Caucasian
Storage Conditions
liquid nitrogen vapor phase
Derivation
The cell line was established from a patient who had received prior chemotherapy and radiation therapy. 
Clinical Data
55 years
Caucasian
female
The patient was a smoker (80 pack years).
Complete Growth Medium
The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001.To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 5%
Subculturing

Volumes used in this protocol are for a 75 cm2 flask; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes.

  1. Remove culture medium, which contains suspended cells, to a centrifuge tube.
  2. Briefly rinse the cell layer with Ca++/Mg++ free Dulbecco's phosphate-buffered saline (D-PBS) or 0.25% (w/v) Trypsin-0.53 mM EDTA solution to remove all traces of serum, which contains trypsin inhibitor.
  3.  Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 10 minutes).

Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach.  Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.

  1. Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting. 
  2. To remove trypsin-EDTA solution, transfer cell suspension to the centrifuge tube containing the medium and cells from step #1 and centrifuge at approximately 125 x g for 5 to 10 minutes.
  3. Discard supernatant and resuspend cells in fresh growth medium.  Add appropriate aliquots of cell suspension to new culture vessels.
  4. Incubate cultures at 37°C.

Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:3 is recommended

Medium Renewal: Every 2 to 3 days. 

Cryopreservation
Freeze medium: Culture medium, 92.5%; DMSO, 7.5%
Storage temperature: liquid nitrogen vapor phase
Culture Conditions
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37°C
STR Profile
Amelogenin:X
CSF1PO:12,14
D13S317:11
D16S539:10
D5S818:10,12
D7S820:9,10
THO1:9.3
TPOX:8
vWA: 16
This cell line was isolated from the same patient as was CRL-5887.
Name of Depositor
AF Gazdar, JD Minna
Deposited As
Homo sapiens
Year of Origin
January, 1988
References

NCI-Navy Medical Oncology Branch Cell Line Supplement. J. Cell. Biochem. suppl. 24: 1996.

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