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NCI-H2087 [H2087]

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編號:B165311

品牌:Mingzhoubio

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DNA

RNA

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產品名稱

NCI-H2087 [H2087]

商品貨號

B165311

Organism

Homo sapiens, human

Tissue

lung; derived from metastatic site: lymph node

培養基

Product Format

frozen

Morphology

epithelial-like and/or rounded

Culture Properties

adherent

Biosafety Level

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Disease

stage 1, adenocarcinoma; non-small cell lung cancer

Age

69 years

Gender

male

Ethnicity

Caucasian

Storage Conditions

liquid nitrogen vapor phase

Derivation

The line was established in November 1988 from a lymph node metastasis of a lung adenocarcinoma.

Clinical Data

69 years

Caucasian

male

The patient was a smoker

Sixty packs year

Comments

A lymphoblastoid line from the same patient is available as ATCC® CRL-5965 (NCI-BL2087).

Complete Growth Medium

The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001.To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 5%

Subculturing

Volumes are given for a 75 cm² flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.

Remove and discard culture medium.
Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution.
Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
Add 6.0 to 8.0 mL of RPMI 1640 medium supplemented with 5% fetal bovine serum, aspirate cells by gently pipetting and transfer to a centrifuge tube. Spin at 125 x g for 5 to 10 minutes. Discard supernatant.
Resuspend the pellet in RPMI 1640 medium and dispense into new flasks.
Incubate cultures at 37°C.

Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:4 is recommended

Medium Renewal: Add fresh medium twice weekly

Cryopreservation

Freeze medium: RPMI 1640 medium, 85%; fetal bovine serum, 10%; DMSO, 5%

Storage temperature: liquid nitrogen vapor phase

Culture Conditions

Atmosphere: air, 95%; carbon dioxide (CO₂), 5%

Temperature: 37°C

STR Profile

Amelogenin: X

CSF1PO: 12

D5S818: 11

D13S317: 12

D7S820: 8, 10

D16S539: 9, 11

THO1: 7, 9

TPOX: 11

vWA: 17

Name of Depositor

AF Gazdar, JD Minna

Deposited As

Homo sapiens

Year of Origin

November, 1988

References

NCI-Navy Medical Oncology Branch Cell Line Supplement. J. Cell. Biochem. suppl. 24: 1996.

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