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NCI-H2110 [H2110]

規格:

價格:

編號:B165315

品牌:Mingzhoubio

標準菌株

定量菌液

DNA

RNA

規格:

凍干粉

斜面

甘油

平板

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產品名稱

NCI-H2110 [H2110]

商品貨號

B165315

Organism

Homo sapiens, human

Tissue

lung; derived from metastatic site: pleural effusion

培養基

Product Format

frozen

Morphology

epithelial-like

Biosafety Level

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Disease

non-small cell lung cancer

Derivation

The line was established in December 1988 from a pleural effusion metastasis of a lung.

Clinical Data

The tissue donor was a non-smoker.

Complete Growth Medium

The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, ATCC 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum (ATCC 30-2020) to a final concentration of 10%.

Subculturing

Remove medium and rinse with 0.25% trypsin, 0.03% EDTA solution. Remove the solution and add an additional 1 to 2 mL of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37°C) until the cells detach. Add fresh culture medium, aspirate and dispense into new culture flasks.

Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:6 is recommended
Medium Renewal: Every 2 to 3 days

Cryopreservation

culture medium 95%; DMSO, 5%

Culture Conditions

Temperature: 37°C

Name of Depositor

AF Gazdar, JD Minna

Deposited As

Homo sapiens

Year of Origin

1988

References

NCI-Navy Medical Oncology Branch Cell Line Supplement. J. Cell. Biochem. suppl. 24: 1996.

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