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NCI-H2141 [H2141]
NCI-H2141 [H2141]
規格:
價格:
編號:B165319
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱
NCI-H2141 [H2141]
商品貨號
B165319
Organism
Homo sapiens, human
Tissue
lung; derived from metastatic site: lymph node
培養基
Cell Type
lymphoblast
Product Format
frozen
Culture Properties
suspension
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Disease
stage E, carcinoma; small cell lung cancer
Age
58 years
Gender
male
Ethnicity
Caucasian
Storage Conditions
liquid nitrogen vapor phase
Karyotype
3p is normal
Derivation

The line was established in March 1989 from lymph node, a metastatic site. 

A lymphoblastoid line from the same patient is available as ATCC CRL-5955.
Clinical Data
The patient received prior chemotherapy and radiation therapy.
The patient was a smoker.
50 pack years
Comments
A lymphoblastoid line from the same patient is available as ATCC CRL-5955.
Complete Growth Medium
HITES medium supplemented with 5% fetal bovine serum
    The base medium for this cell line is ATCC-formulated DMEM:F12 Medium Catalog No.30-2006. To make the complete growth medium,add the following components to the base medium
  1. 0.005 mg/ml Insulin
  2. 0.01 mg/ml Transferrin
  3. 30nM Sodium selenite (final conc.)
  4. 10 nM Hydrocortisone (final conc.)
  5. 10 nM beta-estradiol (final conc.)
  6. extra 2mM L-glutamine (for final conc. of 4.5 mM)
  7. 5% fetal bovine serum (final conc.)

Subculturing
Cultures can be maintained by the addition of fresh medium or alternatively cultures can be established by centrifugation with subsequent resuspension in fresh medium. Cultures grow as floating aggregates of round cell clusters. Single cells are dead and clusters are viable.
Medium Renewal: Add fresh medium as cell density increases.
Cryopreservation
Culture medium, 95%; DMSO, 5%. Cell culture tested DMSO is available as ATCC Catalog No. 4-X.
Culture Conditions
Atmosphere: 5% CO2 in air 
STR Profile
D5S818: 12
D13S317: 14
D7S820: 10, 12
D16S539: 8
vWA: 19
TH01: 6, 8
TPOX: 8, 9
Amelogenin: X Y
CSF1PO: 11
Name of Depositor
AF Gazdar, JD Minna
Deposited As
Homo sapiens
Year of Origin
1989
References

NCI-Navy Medical Oncology Branch Cell Line Supplement. J. Cell. Biochem. suppl. 24: 1996.

Giaccone G, et al. Neuromedin B is present in lung cancer cell lines. Cancer Res. 52: 2732s-2736s, 1992. PubMed: 1563005

Hay, R. J., Caputo, J. L., and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC.

Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988.

Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC.

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