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NCI-H2195 [H2195]
NCI-H2195 [H2195]
規(guī)格:
價(jià)格:
編號(hào):B165322
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱
NCI-H2195 [H2195]
商品貨號(hào)
B165322
Organism
Homo sapiens, human
Tissue
lung; derived from metatstatic site: bone marrow
培養(yǎng)基
Cell Type
lymphoblast
Product Format
frozen
Culture Properties
adherent
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Disease
stage E, carcinoma; small cell lung cancer
Age
67 years
Gender
male
Ethnicity
Caucasian
Storage Conditions
liquid nitrogen vapor phase
Derivation
The line was established in June 1989 from bone marrow metastasis of a lung.
Clinical Data
67 years 
Caucasian
male
Comments
A lymphoblastoid line from the same patient is available as ATCC CRL-5956.
Complete Growth Medium
HITES medium supplemented with 5% fetal bovine serum
    The base medium for this cell line is ATCC-formulated DMEM:F12 Medium Catalog No.30-2006. To make the complete growth medium,add the following components to the base medium
  1. 0.005 mg/ml Insulin
  2. 0.01 mg/ml Transferrin
  3. 30nM Sodium selenite (final conc.)
  4. 10 nM Hydrocortisone (final conc.)
  5. 10 nM beta-estradiol (final conc.)
  6. extra 2mM L-glutamine (for final conc. of 4.5 mM)
  7. 5% fetal bovine serum (final conc.)

Subculturing
  1. Remove medium, rinse with 0.25% (w/v) trypsin - 0.53 mM EDTA solution
  2. Add 1 to 2 mL additional trypsin solution and allow flasks to remain at room temperature (or incubate at 37°C) until cells detach.
  3. Resuspend pellet in fresh medium and dispense into a culture flask.
Subcultivation Ratio: A ratio of 1:2 to 1:3 is recommended
Medium Renewal: Twice weekly
Cryopreservation
Freeze medium: Culture medium, 90%; DMSO, 5%; FBS ,5%
Storage temperature: liquid nitrogen vapor phase
Culture Conditions
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37°C
STR Profile
Amelogenin: X,Y
CSF1PO: 11
D13S317: 11
D16S539: 10
D5S818: 12
D7S820: 10,11
THO1: 9.3
TPOX: 8,9
vWA: 18
Name of Depositor
AF Gazdar, JD Minna
Deposited As
Homo sapiens
Year of Origin
1989
References

NCI-Navy Medical Oncology Branch Cell Line Supplement. J. Cell. Biochem. suppl. 24: 1996.

100 pack years

The line was established from tissue taken from a patient with a 100 pack-year history of smoking.

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